CANNABIS DNA PROJECT

Tom Hill · Aug 23, 2015

i am just now seeing this how is it going?

Tom Hill · Aug 23, 2015

i have a bunch of old seeds too but i want live cells to be restored to viable germplasm in return for my efforts.

Sam_Skunkman · Aug 23, 2015

Great we want them for sure.
-SamS

Londinium said:
Hi Sam,I only just saw this thread as I've been away from IC and the web in general for a while again(usual reasons-I won't bore you). If you are interested I have more dead-collected seed's than I like to be reminded of TBH. I'm not back at my place until next week,but I will gladly sort through my pips then and let you know what's interesting:
I definitely have a bunch of African lines I collected(1 from Transkei I think you might find interesting especially),2 from Brazil,lots of Thai's and other Asian lines old and recent,1 oldie from Mysore and 1 from near Kerala,an Afghan family strain(sent to me) from '81 that's not germinated ever,a couple of old Jamaican lines.......There's other stuff too,I will message you when I have a better Idea and see if they are useful. JBo

Sam_Skunkman · Aug 23, 2015

Well Tom,
To be honest we are not even trying to do that.
I have not even considered it.
We prefer to not have to grow any seeds, we just want dead seeds, to extract the DNA from, or a dry leaf as it is better for us to use.
The best is actually a fresh live leaf but by the time I get them in the post they are molding and unless someone hands me a fresh picked leaf I can put in a freezer immediately I don't want them.
So best is a dry leaf, next best a live seed, then a dead seed. We have not yet done male pollen, but will, and some resin samples from Kazakhstan that we will test the plants cells in with the resin for their DNA.
Sorry I can't help. If you have lots of dead seeds of any one variety send us one or two seed of each variety, that is all we need.
-SamS

TomHill said:
i have a bunch of old seeds too but i want live cells to be restored to viable germplasm in return for my efforts.

Sam_Skunkman · Aug 23, 2015

We have analyzed the first 1000 samples from the USA, mostly poly multi hybrids, I have collected another few hundred samples we have started to extract the DNA and analyze them. In a week or so we should have the first 1000 posted on a public www site.
I am much more interested in the landraces as that will help with the Cannabis family tree that will reveal Cannabis relationships and evolution. I am not so interested in the Bubba-Kush-OG hybrids and who bred them first, but for many others they do care, or they would like to confirm what they have is what they want.
-SamS

TomHill said:
i am just now seeing this how is it going?

Kush_Kloud · Aug 23, 2015

Good work Sam! I'm so Glad we have people like you to help the spread of cannabis knowledge and science.

KK

jay sus · Aug 23, 2015

THIS IS REALLY APPRECIATED.

SpaceBros. · Aug 23, 2015

Sam_Skunkman said:
We have analyzed the first 1000 samples from the USA, mostly poly multi hybrids, I have collected another few hundred samples we have started to extract the DNA and analyze them. In a week or so we should have the first 1000 posted on a public www site.
I am much more interested in the landraces as that will help with the Cannabis family tree that will reveal Cannabis relationships and evolution. I am not so interested in the Bubba-Kush-OG hybrids and who bred them first, but for many others they do care, or they would like to confirm what they have is what they want.
-SamS

Cool. So how many chromosomes in Cannabis? And it's ploidy level, two I suppose? What frequency of samples have different ploidy levels? And monosomies and trisomies ? And how much heteroplasmy do you find in the samples tested? More importantly have you found the hypothetical locus A yet? Oh so many questions. You should probably just give me a job

Can't wait to see those results.

Londinium · Aug 23, 2015

Ok sam,will do....

Londinium · Aug 23, 2015

Hi Tom,hope life's good.Just wondering is that possible now then....Not Sam doing it,but I'm just asking if the process is viable now? I too have a lot of seed that won't germ from lines no-one has and it would be a dream to get some of them back into the genepool

Londinium · Aug 23, 2015

double post

Lester Beans · Aug 26, 2015

Fascinating work, super stoked for the results!

Best!

Tom Hill · Aug 26, 2015

Londinium said:
Hi Tom,hope life's good.Just wondering is that possible now then....Not Sam doing it,but I'm just asking if the process is viable now? I too have a lot of seed that won't germ from lines no-one has and it would be a dream to get some of them back into the genepool

It is absolutely doable being done and save those seeds brother they will live again!.

Tom Hill · Aug 26, 2015

Sam_Skunkman said:
We have analyzed the first 1000 samples from the USA, mostly poly multi hybrids, I have collected another few hundred samples we have started to extract the DNA and analyze them. In a week or so we should have the first 1000 posted on a public www site.
I am much more interested in the landraces as that will help with the Cannabis family tree that will reveal Cannabis relationships and evolution. I am not so interested in the Bubba-Kush-OG hybrids and who bred them first, but for many others they do care, or they would like to confirm what they have is what they want.
-SamS

I have some landrace seed sam right up this alley i will see that they get to you..

purple_man · Aug 26, 2015

done know tomz

glad to see you back online...

@sams: could you please let ICMAG know, when the data goes online (thx alot in advance)??? also, i hope you guys stored unused "unviable" seeds, embryo rescue and in-vitro culture could prove VERY usefull if the sequenced line is one of the "building blocks which were lost"....

blessss

Tom Hill · Aug 26, 2015

trust me when i say they will tuck a few away for later research tehee

Tom Hill · Aug 26, 2015

just anti up and pay the man ha? We owe him and his colleges and they'll likely do more for cannabis than we would with the same given opportunity.

purple_man · Aug 26, 2015

i hope so for them

as the wu tang clan would say: C.R.E.A.M ... one thing is for sure, lots of $ could be possibly generated with that kind of data

blessss

ps.: pay the man for what actually? he is doing the PR stunt "we will share the data with the public" so folks send in more stuff, and now we should pay for it??? screw that, no freaqin company ESPECIALLY not a "biotech" startup having sams involved in times like these is an act of kindness to mankind and the cannmunity, it's just a way to get payed, and stay on "top of the game"....

pps.: im stil more then glad that they are doing what they are doing, even if the data gets disclosed in 15 years or folks can get access via payment

Tom Hill · Aug 27, 2015

if u really must have an answer then pay the man for the fuel he provided to burn the very flames on which this fire burns lol.. hey man u saw me try to get something in return ha?

but i'll give anyway,, because just like u whether u know it or not,, we owe..

Tom Hill · Aug 27, 2015

back away from it and tell me what exactly is possible for you to do purple man,? what you got a poster to donate to some museum and that is worth something? Are you fucking kidding me? These guys get shit done they been getting shit done for eons this is not Sam this is Sam and his colleges and i assure you there is no mother fucker in the world who has ever done or has plans to do anything better,, in-spite of what is going on inside their little monkey brains lol..

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