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Old 02-25-2017, 08:07 AM #1
Tonygreen
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Let's make Double Haploids

I see more and more people with the knowledge and skill set around here. I am still working on my aseptic protocol skills.

So what's up this is the double hap thread.
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Old 02-25-2017, 11:03 AM #2
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Care to explain what a double haploid is?
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Old 02-25-2017, 01:05 PM #3
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Colchicine?
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Old 02-25-2017, 02:51 PM #4
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Double haploid = diploid = normal organism

You sure you're not trying to make a tetraploid or triploid?
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Old 02-28-2017, 07:46 AM #5
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A double haploid is the fastest way to a true homozygous true breeding plant.

I have some protocols that also use oryzalin which may be much easier to source than colchicine.

I am truly surprised we have not seen this in cannabis. I seen a guys thread with a nice lab set up making tetras and tris... to me thats worthless, perfect set up for making double haps too!

True breeding in 1 generation.

I am eyeing a professional flow hood, I made one but it sucks ass ha!
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Old 02-28-2017, 07:53 AM #6
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Quote:
Originally Posted by Rox View Post
Care to explain what a double haploid is?
doubling of chromosomes in a haploid cell makes a double hap.
A way to strongly influence probability in our favor...
The ability to make a homozygous line in one round can literally save a breeder a lifetime of work.
Traditional breeding methods are slow, one could fight heterozygosity for years trying to develop a strain. the big deal is more elite can be created and selected within less time.
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Old 02-28-2017, 03:12 PM #7
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Quote:
Originally Posted by Tonygreen View Post
doubling of chromosomes in a haploid cell makes a double hap.
A way to strongly influence probability in our favor...
The ability to make a homozygous line in one round can literally save a breeder a lifetime of work.
Traditional breeding methods are slow, one could fight heterozygosity for years trying to develop a strain. the big deal is more elite can be created and selected within less time.
Pics or it didn't happen.
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Old 03-01-2017, 03:54 AM #8
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I am eyeing a professional flow hood, I made one but it sucks ass ha!

I saw a youtube vid from this guy who does plate swaps
for failed hard drives and his rig is self made and true.

I'll look around for the link and get back to this thread and post.
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Old 03-11-2017, 11:17 PM #9
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Interesting subject!
Not sure how good that strategy will pay off with cannabis, though. There might be simple protocols which 'accidentally' work on cannabis too but what do you do with a doubled haploid then? It will most likely be a fragile, sick, weak, crippled runt whose only sense in life is being a homozygous carrier of a valuable and/or new mutation which will only come into play once outcrossed. To me right now (I admit, it's been a long day for me) it seems less trouble (and certainly more fun) to create a doubled haploid just because you can than all the crossing with other doubled haploids needed to find the real goal, a 'perfect' F1. Without genetic markers, the value of a DH especially in outcrossing species seems rather low to me.

I was reading into that subject a while back and there's a bunch of feasible strategies for certain crops. Most often, it's a freak of nature or a coincidence which creates and/or rescues the haploids of a given plant species (for example wide crossing in barley or tobacco: doesn't work so far with cannabis x hops, or does it?). Colchicine and IIRC also caffeine are used in the second step where you double the chromosomes. You should probably focus on gyno- and especially androgenesis . If I can re-find those papers again, I'll let you know.
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Old 03-21-2017, 01:25 AM #10
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Quote:
Originally Posted by Tonygreen View Post
doubling of chromosomes in a haploid cell makes a double hap.
A way to strongly influence probability in our favor...
The ability to make a homozygous line in one round can literally save a breeder a lifetime of work.
Traditional breeding methods are slow, one could fight heterozygosity for years trying to develop a strain. the big deal is more elite can be created and selected within less time.
High Tony

I've been trying to get my head around this & my head is spinning managed to get as far as understanding that a double haploid is a diploid as it carries 2 sets of chromosomes..Yeah? Or is a double haploid something else?

So if i am right, a diploid is identical to its parent? A haploid is a combination of both but not unique to either parent..

So if this diploid under goes Apomixis then..BINGO! Instant homozygous seeds...

I'm so excited at this prospect, assuming I have understood this correctly?

Anybody care to share some pictures?

Thanks
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