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Old 04-07-2011, 02:24 AM #41
Microbeman
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Originally Posted by GrowGeek View Post
Chimera, I have thought of selling my tv, but not my bed. I have (however) cut back on my scotch intake... Bumped it back to 12yr from 20yr! ah ha ha ha!

I'm slowly getting my culture lab setup. Saving pennies where I can.
I just did the opposite...thinking f* it. only live once.... may as well drink Glenfiddich...well unless I figure out how to transfer my mind to this 15 year old clone I have in the tank.
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Old 04-07-2011, 05:58 PM #42
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Shoot Culture is neat and all but pretty basic TC wise.

The real fun comes with callous culture and shoot regenation. Callous is the real 'stem cells' or totipotent cells in plants... using callous and regenerating shoots opens up all sorts of possibilities.

Coming soon to a med state/country near you!

-Chimera
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I'd love to see your babies get around

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Old 04-26-2011, 07:52 PM #43
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The frogs eggs are a done deal Imona; the only barrier to making these available across the board is a legal one... no current seed sellers are willing to list them as they don't fit the definition of a seed... so the retailer would essentially be violating laws by 'trafficking in cannabis'. Makes international shipping and distribution... problematic.
it would still be only whatever the plant material dried weighed..so 10 tissue culture samples dried would be like what less then an 1/8th? i don't see that being a huge deal with domestic distribution. international and customs may be a different story though.
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Old 04-28-2011, 01:29 AM #44
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use ebay for flowhoods/hepa's. one can set up a full(non pro) lab for around 1k room permitting.

good site that aggregates ebay hepa's:
https://www.fungifun.org/English/Flowhood

I picked up several 2'x2'x10" last year for $50 a piece.
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Old 05-05-2011, 08:30 AM #45
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I purchased a home based TC kit from a supplier via eBay which cost me about $40 with the intention of using it to preserve desirable phenos, but have never got around to putting it to use. I'd really love to build a sealed clean room when we build new digs in a few years time.

To be honest the only thing that stopped me was my inability to settle for an upturned box as a hood and difficulty justifying the expense of a laminar flow hood to the commissioner of fun and finance.

Chimera those Frogs eggs certainly sound interesting, do you have a post relating to them?
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Old 05-05-2011, 09:05 AM #46
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Did my first Cannabis TC more then 20 years ago after I self taught myself and bought all the needed lab equipment and set up a small in-vitro lab.
I found it pretty easy to Clone in-vitro meristems, Callus even easier, but I did have trouble getting the Callus to shoot after rooting. Rooting is easy.
Growing meristems and Callus is easy, so is sterilizing a clone so you can stick it in agar and nutrients to grow.
Give it a try, it is easy, and if anyone figures out how to freeze Callus or Meristems and then thaw them out and re-grow them please let me know, that was my real interest in the first place. When I did it they were reduced to black slime lumps, zero life...
As for freezing, I had a professional In-vitro Company try and find a way to freeze meristems or Callus, but after a few years gave up, I still think it will be done by pretreating the Callus or Meristems or other newer protocols in use today that we did not try.
My dream was a library that would maintain varieties or unique individuals in a very small space, for very long times, that could be re-grown if needed. Keeping clones alive is such a pain and waste of time, space and energy. Freezing would be great!!

-SamS
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Old 05-05-2011, 09:09 AM #47
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Very interesting stuff here. Ive been fascinated with the idea of housing 100s of genotypes, maintenance free, taking up little space, and not having to devote an entire room to house them. Ive seen those home kits for ~$200, are these all I would need to get started? they sell kits for african violets, would these kits contain the same hormones and things I would need to take callous cultures from cannabis
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Old 05-05-2011, 10:46 AM #48
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The Revolution, as I understand it (and that's not widely) different cells will react to different hormones depending on concentration and type, and it's all a bit of trial and error.

eBay tissue culture kit and you'll get a few hits
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Old 05-05-2011, 11:14 AM #49
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Very interesting info.
Thank you to all you guys.
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Old 05-07-2011, 11:04 PM #50
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Quote:
Originally Posted by Sam_Skunkman View Post
As for freezing, I had a professional In-vitro Company try and find a way to freeze meristems or Callus, but after a few years gave up, I still think it will be done by pretreating the Callus or Meristems or other newer protocols in use today that we did not try.
-SamS

Did they try the standard PEG/DMSO? I think this method came into common use in the mid '80s- if your efforts were before this, I would try it, I assume that it is pretty effective for most plants. I think they sometimes create a suspension of callus cells and freeze that, I've heard that this helps freeze the cells very quickly, which is important.

Patent deposits of TC at ATTC are required to be frozen callus, so it must be the standard way of preserving TC?

I know it works really well for beer yeast cultures.
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