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Old 11-12-2018, 07:13 AM #131
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Quote:
Originally Posted by Darpa View Post
Hi folk,
I’m looking for an anther culture and callus induction medium formulation. I’m thinking about testing:

Murashige and Skoog’s medium containing 3% (w/v) sucrose, 0.8% Type E agar supplemented with 0.5µM naphthalene acetic acid (NAA) in combination with 1.0µM of thidiazuron (TDZ)

Murashige and Skoog’s medium containing 3% (w/v) sucrose, 0.8% Type E agar supplemented with plant growth regulators: BAP (1mg/l) and NAA (0.5mg/l)

Any other suggestion would be greatly appreciated.

Darpa
I'd try equal amounts of cytokinins and auxins to initiate a callus.
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Old 11-16-2018, 09:14 PM #132
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Quote:
Originally Posted by Darpa View Post
Hi folk,
I’m looking for an anther culture and callus induction medium formulation. I’m thinking about testing:

Murashige and Skoog’s medium containing 3% (w/v) sucrose, 0.8% Type E agar supplemented with 0.5µM naphthalene acetic acid (NAA) in combination with 1.0µM of thidiazuron (TDZ)

Murashige and Skoog’s medium containing 3% (w/v) sucrose, 0.8% Type E agar supplemented with plant growth regulators: BAP (1mg/l) and NAA (0.5mg/l)

Any other suggestion would be greatly appreciated.

Darpa
Ebay has a product medium made in germany for sativa plants pre mixed
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Old 11-17-2018, 06:00 AM #133
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with DNA and bubbleman behind the tissue culture company here in canada. anyone know if tissue cultured plants could be considered organic since grown with salts and PGRs for part of there life.. esp TDZ and the cytocides to prevent contam maybe not alot makes it into the plant but likely not super awesome for concentrates? think certified organic would def be off the table?
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Old 11-17-2018, 09:49 AM #134
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[quote=Chimera;4342684]
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Originally Posted by Chimera View Post
04-01-2011, 06:06 AM

Shoot Culture is neat and all but pretty basic TC wise.

The real fun comes with callous culture and shoot regenation. Callous is the real 'stem cells' or totipotent cells in plants... using callous and regenerating shoots opens up all sorts of possibilities.

Coming soon to a med state/country near you!

-Chimera

G `day Chim

Define soon ?

Thanks for sharin

EB .
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Old 11-20-2018, 03:42 AM #135
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Quote:
Originally Posted by clearheaded View Post
with DNA and bubbleman behind the tissue culture company here in canada. anyone know if tissue cultured plants could be considered organic since grown with salts and PGRs for part of there life.. esp TDZ and the cytocides to prevent contam maybe not alot makes it into the plant but likely not super awesome for concentrates? think certified organic would def be off the table?
Hi Clearheaded, I haven't do an extensive check up on the subject, but it seem that tissue cultures plants wouldn't be an issue for a biologic certification. (at least under Canadian regulation).


Natural PGR are autorized (sorry it's in french)
Régulateurs de croissance des plantes: Les hormones végétales naturelles, comme l’acide gibberellique, l’acide indole-acétique (AIC) et les cytokinines, sont permises. Voir aussi Acide gibberellique.

Therefore, TDZ, wich is a citokinin, is autorized...

Ref: List of autorized substances for biologic production
https://publications.gc.ca/collectio...1-2006-fra.pdf
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Old 11-20-2018, 11:52 PM #136
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there are a few of the needed PGRs that are considered safe and organic. your right. however several/many are not. TDZ is has a small amount of toxicity(mostly to fish) and is infact banned in the EU. while of course biological mechanisms are different in fish and humans to me it means it should be avoided as something that will be inhaled. like pyrethrin. "ok" to eat however inhaling is a little different and also toxi to fish. also TDZ is not organic and 100% synthetic compund. just because a chemical falls into a class that is naturally occuring does not make it organic or safe...

Also because 90% of PGR are synthetic and infact not organic I do find it hard to believe. As PGR are used for cotton and one of the reasons people shy away from non organic cotton. does organic cotton still use the PGRs before harvest??

1 other point which would prevent them from being organic is of course they are grown on salt based mediums. how could a TC plant become organic because its grown on slat based nutes? antiiotics antifungals in the medium is common aswell to produce in mass. are those something you want to smoke? are they considered organic even if antibiotics may be gotten from fungus?? organic beef use organic antibiotics???


Pushkar Kale, Agricultural and Food Engineering, IIT Kgp
Answered Mar 17, 2016 · Author has 206 answers and 322.7k answer views
"In case of Tissue Culture, due to use of artificial media, it is prohibited."

So my sense is while seed potatos are started in tissue culture they are grown out and then those potatoes are then the seed potato ie generation 1year away. they can be organic because are not grown inorganic medium. however a cannabis plant started in inorganic salts would not be considered organic..

Also if use your logic of PGR OK because bsed on organic structures but synthetically produced or tottaly new chamical. Can plants then be sprayed with PGRs and be considered organic??

love to hear chimera chime in.
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Old 11-21-2018, 01:44 AM #137
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Hi Clearheaded,

After further verification, you are wright. It would be very difficult to get a biological certification if you are starting with tissue culture material. Synthetic growth regulator are not authorized under Canadian Organic certification (I missed that note regarding section 4.1.3: Substances listed in Tables 4.2 and 4.3 shall comply with prohibitions in 1.4 of CAN/CGSB-32.310.):

CAN/CGSB-32.310, Organic Production Systems – General Principles and Management Standards, describes the principles and management standard of organic production systems.

CAN/CGSB-32.311, Organic Production Systems – Permitted Substances Lists, provides lists of substances that are allowed for use in organic production systems.
General Principles and Management Standards.

CAN/CGSB-32.310-2015 Amended March 2018

1.4 Prohibited substances, materials or techniques in organic production and preparation If producing or preparing organic products, the following substances, materials or techniques are prohibited since they are incompatible with the general principles of organic production:

a) all products of and materials from genetic engineering (GE), as defined in this standard, and as specified in 4.1.3, 5.1.2 and 6.2.1 of CAN/CGSB-32.311;

b) all products, materials or processes intentionally using nanotechnology, as defined in this standard, with the following exceptions:

1) naturally occurring nano-sized particles or those produced incidentally through processes such as grinding flour;

2) contact surfaces, such as equipment, work surfaces, or packaging, where transference of nano-sized particles to organic crops, livestock or products is unintended and unlikely to occur;

c) irradiation, as defined in this standard, for the treatment of organic products and inputs used in the production of organic products, except as specified in CAN/CGSB-32.311;

d) soil amendments, such as fertilizer or composted plant and animal material, that contain a substance not listed in CAN/CGSB-32.311;

e) sewage sludge;

f) synthetic crop production aids and materials, except as specified in CAN/CGSB-32.311;

g) synthetic growth regulators;

h) cloned livestock and its descendants;

i) synthetic allopathic veterinary drugs, including antibiotics and parasiticides, except as permitted by this standard;

j) synthetic substances used in organic product preparation, such as ingredients, food additives and processing aids, including sulphates, nitrates and nitrites, except as specified in CAN/CGSB-32.311;

k) equipment, harvest and storage containers, storage facilities and packaging materials treated with synthetic fungicides, preservatives, fumigants and pesticides;

l) substances that are not listed in CAN/CGSB-32.311, except as permitted in this standard.
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Old 11-21-2018, 02:45 AM #138
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That been said, Certified Organic or non-certified organic products, it’s just a marketing tool in order to differentiate your product on today market. There is no health concern about consuming non certified organic product… But if you prefer bio, just go with it….
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Old 11-21-2018, 03:18 AM #139
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It exists for a reason, some reason. To say it is just a marketing tool and there is no health concern does not sound entirely comprehensive/accurate. Tissue culture may be ok to use right away or after 1-2 generations but your post is dismissive of organic standards.
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Old 11-23-2018, 08:01 AM #140
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Thanks for clearing that up Darp.

speaking of marketing Bman is convinced it is organic buut think he just doesnt know enough, but thats on him, everyone is free to go through this world as they like, just a little let down he didnt read more as they will be pushin this potentially laced stuff to potentially all/most large LPs (at least if is found to be viable, which is yet to be seen). whats worse is for it to be viable it isnt going to be for moms it will be 1000s of plants to be flowered. So much talk on hash church about clean products and issue with even using clones of clones that are sprayed failing testing...

Well while "organic" has a life of its own pun intended, as far as marketing and meanings to people. may not cover all we would like or what BIO is in euro. there is certainly more then marketing in organic herb or organic tomato, once you experience the difference its hard to deny and more then sugar content. Also it is making a choice to reduce chemicals in environment not only leakage but manufacture/transport and also those chems in your body. once you get older it seems the folks who sprayed there lawns and didnt bother washing things seem to be the ones getting cancer 100% anectdote, but after being exposed to multiple things over the years that we are told is "OK" then being told its not 15-20 years later and taken off the market, makes sense it would add up. What were we talking about again?? lol anyway. long story short unfortunete TC isnt organic..

tried to get chimera to chime in hopefully he does, not sure if he invested or not. feel like he would of held off as knows there is no real barrier to entry.
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