I
icon
just incase anyone is interested chimera made a thread on freezing pollen https://www.icmag.com/ic/showthread.php?t=228252
-
Happy Birthday ICMag! Been 20 years since Gypsy Nirvana created the forum! We are celebrating with a 4/20 Giveaway and by launching a new Patreon tier called "420club". You can read more here.
-
Important notice: ICMag's T.O.U. has been updated. Please review it here. For your convenience, it is also available in the main forum menu, under 'Quick Links"!
HOW OLD CAN POLLEN BE ALIVE?
- Thread starter Sam_Skunkman
- Start date
l337purpl3
Member
-30 degrees!!! Would those be those special freezers they keep semen in? Was trying to get more info on those freezers for breeding purposes (dogs)
It is a big lab freezer, I will have to check. I have several, some for more then 20 years now.
-SamS
-SamS
budseyeveiw
Member
wow you get hi tech with this whole storage thing. i stuck mine on a shelf and wondered if its viable a few months later... probably not lol
Sam many thanks, I have been wondering about the self defrosting freezers harming pollen as I keep some of mine in one that does. The last time I tried some of it I was unsucsessful..though I'm hit or miss most of the time anyway. Cheers..DD
Yes tetrazolium can be good although it only enables you to check whether you have viable cells but not if your pollen can germinate. If you work with big quantities you do increase your chances to get viable seeds even if the pollen is poor quality. I must say I never tried keeping cannabis pollen using these techniques, only used fresh pollen from males i'd test before and kept as clones but I work everyday with pollen from oil palm (know to be rather resistant as its a insect pollinated specie) that are kept that way and it works great. Lately we had some pollen sent from Africa which had a problem during shipping (even if well kept before) and germination rate was around 40% (loss of more of less 50% viability in a 15h trip!).
bonbolos,
What is the pollinator of the Coco de Mer?
I imported a few dozen into Kauai, 20 years ago alive as coconuts and some grew fine.
They are getting old enough to show sex soon, hope I have both a girls and boys.
Any ideas what does the deed?
-SamS
What is the pollinator of the Coco de Mer?
I imported a few dozen into Kauai, 20 years ago alive as coconuts and some grew fine.
They are getting old enough to show sex soon, hope I have both a girls and boys.
Any ideas what does the deed?
-SamS
audioaddict
Active member
This still isn't fully understood, but the best educated guesses are Lizards, or just the elements (wind and rain)
i have a defrosting type freezer, kept my pollen good for about 2 years, but that's as far as it would go, 2 failed pollinations after that, so 2 years in the standard freezer?
Geckos are very unlikely to be the pollinator animal. I'm no specialist but after a little research you can find this very good article explaining a lot on the subject (available in PDF) http://www.hindawi.com/journals/jb/2012/687832/abs/
is it a theoretical question?
if i remember right, the coco de mer is pollinated by geckos...
and pollen stays viable as long as the environmental factors allow it, same goes for seeds...
another thing would be, if hypothetically one deep freezes prepared tissue of a male, which can be thawed and regenerated, acclimatized and utilized as a fresh pollen donor? or straight dehydrating the pollen to a certain humidity content and deep freezin it? 10 + years at least, if the cooling "chain" can be ensured
blessss
ps.: stop teasing sam
let us know whats YOUR oldest viable protocol? please!
if i remember right, the coco de mer is pollinated by geckos...
and pollen stays viable as long as the environmental factors allow it, same goes for seeds...
another thing would be, if hypothetically one deep freezes prepared tissue of a male, which can be thawed and regenerated, acclimatized and utilized as a fresh pollen donor? or straight dehydrating the pollen to a certain humidity content and deep freezin it? 10 + years at least, if the cooling "chain" can be ensured
blessss
ps.: stop teasing sam
let us know whats YOUR oldest viable protocol? please!I started my own thread on a similar topic when I got inspired by this one. Everyone thinks the topics are one in the same I guess, I got no real answers. So forgive me, but I will ask here: how long can pollen remain viable in dried flowers? In my case, I am worried about accidental pollination. Can pollen released from a flower drying for 1+ month last in the air another month and create the undesired effect of pollinating clones that will be in flower by then?
Atlantic Allele
Member
Impressive.
I'm surprised freezing it didn't kill the cells through ice crystals. 17 years is a long time!
quick question. if i put a platic bag over my male then transfer it into a different bag after getting rid of other plant material,allow it to dry would i then be able to put the bag in a foodsaver vac to get rid of unwanted air would this work. also im curiouse to how the gel caps worked.
thanks in advance
thanks in advance
Mrgreenharvest
Member
what about non storage? if i did some breeding in a room, killed the male and pollen remained in the room, how long would that pollen stay alive?
would 1 week after be a safe measure if i was moving more plants in?
would 1 week after be a safe measure if i was moving more plants in?
