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Old 09-06-2017, 11:18 PM #1
ReikoX
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Ancestral Skunk #1 - Grow Show

I got a couple packs of these to hunt through. I'm starting with about 50 of these seeds this round. As you can see these seeds are quite dirty.


Chimera was kind enough to post his protocol for popping old seeds.

Quote:
Originally Posted by Chimera
The protocol I use is pretty simple.

5% bleach in autoclaved, distilled water with a couple of drops of Tween-20 per liter of solution. I sterilize the water in Duran jars (loose lids) in a pressure cooker @ 20 PSI for 20 minutes, and then allow the jars to cool under my flow hood and tighten the lids when the water has cooled. This is probably overkill for most purposes, but it's a variation of the protocol I use for sterilizing explants for tissue culture, and it seems to work with minimal contamination. You can get away with a drop of dish detergent instead of the Tween, I just have the tween-20 in the lab so I use it. If you are going to be doing a lot, you can order a few ml of Tween-20 on Amazon if you like.

First I rinse the seeds in a tight mesh strainer to remove any leaf debris that might be harboring bacteria or spores. These seeds were a little dirtier than most having been field grown, so you definitely want to wash away any superfluous materials. This can be up to a 5 minute rinse in lukewarm water. Cannabis achenes have a lot of little crevices where spores and bacteria can hide, so a thorough rinse is a wise step.

Then I take the seeds and drop them in maybe 100-200 mls of the bleach/Tween-20 solution in an Erlenmeyer flask, but any clean glass vessel will do -the seeds should be fully immersed, maybe 2x the volume of water as seed. You want to keep stirring and swishing the seeds around gently to ensure the current is washing away any contaminants. After a minute of swishing I remove the bleach and strain the seeds from the liquid and start again. No more than 3 minutes total, and if I see bleaching on the small test batch (always do a test batch to seed how the seeds react), sometimes I'll only do 2 minutes.

The next part is key. Immediately rinse 3-5 times (for 5 minutes per rinse) in the same sterilized distilled water (baby water / distilled water from Walmart or the local pharmacy is fine), again using a tight mesh strainer, or simply rinse the seeds in a different vessel with the same volume of water, at least 3 times to ensure the bleach is removed. I use a labratory orbita shaker, but you can do it by hand it's just a little tedious. Once the rinses are completed, soak the seeds in the distilled water as before, for 2-24 hours depending on the age of the seed and the medium you will be growing in. Sometimes I'll use agar plates, but most often I'll just sprout them in paper towels and transfer to a light dirt (like Light Warrior for you Americans) or surface sow on a .peat based planting substrate. You can use whatever medium you prefer, it's not that big of a deal as long as the seeds remain moist.
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Old 09-06-2017, 11:21 PM #2
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First I needed some sterile water, so I put a few cups of RO water in the bottom of the pressure cooker along with a glass jar and lid. This was cooked @15 psi for 20 minutes. I let this cool and then poured the water into the sterilized jar for later.


Next I made a 5% bleach solution with a drop of detergent. This was also sterilized in the pressure cooker @15 psi for 20 minutes.


The two jars were allowed to cool. Then, I gave them a five minute wash in luke warm tap water through a mesh strainer.


After the rinse, they were put in a glass container and washed with the bleach solution for one minute. The seeds was strained through the strainer and washed two more times. Then, the seeds were put back in the container and rinsed with the sterile water for five minutes. Again, they were strained and rinsed two more times.


Finally they were covered with sterile water and allowed to soak in the cupboard for the next 24 hours.
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Old 09-06-2017, 11:54 PM #3
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for sure in for this one.
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Old 09-06-2017, 11:56 PM #4
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Very nice !
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Old 09-07-2017, 12:29 AM #5
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Pulling up a seat for the show RX
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Old 09-07-2017, 01:25 AM #6
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Mate, you've gone above and beyond the call of duty with your sterilization techniques... thanks for posting that!

(you even sterilized the bleach solution??! above and beyond....)


Go mate, go!
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Old 09-07-2017, 01:34 AM #7
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This one looks interesting!
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Current: the church greenhouse seeds
https://www.icmag.com/ic/showthread.php?t=345592

Get free seeds from skunk24! No purchase necessary!
https://www.icmag.com/ic/showthread.php?t=339578
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Old 09-07-2017, 02:50 PM #8
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Can't wait to see the results but it looks like I'm going to have to.
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Old 09-07-2017, 02:58 PM #9
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This is promising
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Old 09-07-2017, 03:55 PM #10
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Very interesting in many aspects!Good luck!
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