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Old 05-15-2019, 08:30 PM #1
moses wellfleet
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Endophytes for ipm

Please read this article and join a discussion on this topic!

I have a plant that is very resistant to powdery mildew and some cuts that are very susceptible to it!

My plan is to do the whole leaf smoothie thing with the resistant plant and try inoculate the cuts that suffer heavily from pm!

https://blogs.scientificamerican.com/artful-amoeba/leaf-fungus-smoothie-brings-endangered-hawaiian-flower-back-from-the-brink/
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Old 05-15-2019, 08:49 PM #2
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This sounds neat as can be !
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Old 05-15-2019, 09:18 PM #3
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This sounds neat as can be !
That’s what I thought, and it is not so different from inoculating human babies against disease!
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Old 05-16-2019, 01:13 AM #4
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Quote:
Originally Posted by moses wellfleet View Post
Please read this article and join a discussion on this topic!

I have a plant that is very resistant to powdery mildew and some cuts that are very susceptible to it!

My plan is to do the whole leaf smoothie thing with the resistant plant and try inoculate the cuts that suffer heavily from pm!

https://blogs.scientificamerican.com...rom-the-brink/

great article Moses , I didn't see where they stated what the 11 known endophytes they had used were.

They mention yeast but not the others I could see. Still interesting they found PM in such large numbers after the inoculation and the plant was thriving according to them.


I was thinking that your cuts (same mother?) need to be inoculated with soil from that mother. It may be as simple as that but if you already are inoculating, then disregard this message.


If you haven't, Id try that and drink that smoothie
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Old 05-16-2019, 07:54 AM #5
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Yes so I am growing in organic soil that I reuse, replanting directly in the same container after harvest. Some plants would indeed have been exposed to soil from the highly resistant plant. There is one strain that possibly did build up some resistance after arriving, in fact it is the cut that brought the pm in the first place. I am going to run mostly the resistant plant from now on so there will be greater exposure!!

I’m only assuming that endophytes are responsible for the resistance. If it is somehow just naturally genetically resistant to pm then the smoothie wouldn’t work!!
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Old 05-16-2019, 03:05 PM #6
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I have long hypothesized that dark septate endophytes (DSE) are what I observe in ACT and V-compost slurries. Many growers able to produce CT with high fungal populations report highly successful vigorous gardens.

I have maintained that these fungi-imperfecti grow into the root systems, enhancing nutrient uptake and pathogen resistance.

Here is a relatively decent article. Be aware of one error where it is implied that DSE are all melanized [colored]. This a misnomer. Many DSE are hyaline [colorless] and many start hyaline and become melanized.

https://journals.plos.org/plosone/ar...l.pone.0061332

Another good article;

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4408093/

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All DSE strains except A. macrosclerotiorum formed intracellular melanised or hyaline microsclerotia
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Last edited by Microbeman; 05-16-2019 at 03:25 PM.. Reason: another articl
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Old 05-16-2019, 03:58 PM #7
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I've read many reports (the last few years now) of the same clones having differing resistances to PM, with the difference being nutritionally based. I would first make sure the cuts are indeed getting the same nutrition as the donor parent. Using recycled organic without testing may make this more difficult.
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Old 05-16-2019, 04:40 PM #8
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Originally Posted by Douglas.Curtis View Post
I've read many reports (the last few years now) of the same clones having differing resistances to PM, with the difference being nutritionally based. I would first make sure the cuts are indeed getting the same nutrition as the donor parent. Using recycled organic without testing may make this more difficult.
I've seen many reports of diseased hydro plants quarantined in anaerobic earthbox conditions and fully recover from all symptoms.. We could count the microbes, interview them, whatever, but I prefer to simply accept the supremacy of nature. You dont have to think about things when you submit
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Old 05-16-2019, 05:59 PM #9
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Originally Posted by moses wellfleet View Post
Yes so I am growing in organic soil that I reuse, replanting directly in the same container after harvest. Some plants would indeed have been exposed to soil from the highly resistant plant. There is one strain that possibly did build up some resistance after arriving, in fact it is the cut that brought the pm in the first place. I am going to run mostly the resistant plant from now on so there will be greater exposure!!

I’m only assuming that endophytes are responsible for the resistance. If it is somehow just naturally genetically resistant to pm then the smoothie wouldn’t work!!

After reading the 1st paper MM linked to, it makes sense (to me) that your cut reached a happy place in regards to fungal infection. Perhaps your new cuts will eventually do the same given time and the right conditions which include the proper amount of inoculation from the start. The paper refers to it as pattern and quantity.


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Subsequently, the FPDR increased rapidly, suggesting more hyphae colonized within the roots, finally reaching a steady-state level in rice roots, indicating that fungal proliferation was synchronized with root growth. The fungal biomass was restricted to a certain extent without the ingression of additional hyphae from outside or overgrowth of the hyphae inside. This indicates a transition from an unlimited to a limited fungal growth pattern in the host root and homeostasis between root growth and fungal proliferation. In contrast, the pathogen showed unlimited proliferation in roots and even spread to aerial tissues accompanied by a continuous increase in fungal biomass. This fungal overgrowth led to plant disease and killed the host [53]. Therefore, another difference between mutualism and antagonism is quantitative rather than qualitative. Taken together, these observations indicate that colonization pattern and quantity are deciding factors in whether a fungus-plant interaction is mutualistic or antagonistic.
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Old 05-16-2019, 06:54 PM #10
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Originally Posted by Douglas.Curtis View Post
I've read many reports (the last few years now) of the same clones having differing resistances to PM, with the difference being nutritionally based. I would first make sure the cuts are indeed getting the same nutrition as the donor parent. Using recycled organic without testing may make this more difficult.
Any links to those reports?

Would be interesting to see if an overabundance of P played a role in suppressing endophyte colonization, including Mycorrhizal fungi
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