nohnsmith1010
Member
HELLO
so i have had so many conversations about this I thought i wound post about it just to see how wide spread this misconception is .
-So during testing procedure for HPLC the sample ( eg 100g ) is centrifuged for 4 hour with tech grade methanol . The resulting mix is filtered to 1 micron for sampling in the high pressure liquid chromatography unit .
WHEN THE THC % IS GIVEN IT IS ON THE EXTRACTED SAMPLE NOT THE ORIGINAL WEED WEIGHT.
Average oil extracted is 15% of weed weight ( 10% to 20% )
So the 20% THC rating ( thca + thc) is based on that 15% oil not the original weed sample.
the math is:
100 gram weed rated at 20% thc has 15% avg oil extraction at a thc % of 20% .
100g x 15% x 20% = 3 gram in a 100 g weed which makes it 3% thc NOT 20%
this is simply 4he testing procedure .
if weed was really 20% thc then the trichome would have to be 100% thc .
if you extracted the trichomes of avg weed you would be lucky to get 15% trichomes back and you can't have more thc than the gland that makes it .
AMD if you separated it, the resulting resin would have to be 100% thc and be completely free of terpenes and other compounds.
this is a quote from a testing procedure :
Below the HPLC chromatograph are two charts that highlight the cannabinoid potencies of your product. The first chart identifies cannabinoid concentrations (Fig. 3). As you can see, the delta-9-tetrahydrocannabinol (THC) level is 24.64 per cent (the product sample analyzed in this example is a cannabinoid extract, so the concentrations are much higher than what would occur in dried flowers).
I will post sample prep procedures in pics
so i have had so many conversations about this I thought i wound post about it just to see how wide spread this misconception is .
-So during testing procedure for HPLC the sample ( eg 100g ) is centrifuged for 4 hour with tech grade methanol . The resulting mix is filtered to 1 micron for sampling in the high pressure liquid chromatography unit .
WHEN THE THC % IS GIVEN IT IS ON THE EXTRACTED SAMPLE NOT THE ORIGINAL WEED WEIGHT.
Average oil extracted is 15% of weed weight ( 10% to 20% )
So the 20% THC rating ( thca + thc) is based on that 15% oil not the original weed sample.
the math is:
100 gram weed rated at 20% thc has 15% avg oil extraction at a thc % of 20% .
100g x 15% x 20% = 3 gram in a 100 g weed which makes it 3% thc NOT 20%
this is simply 4he testing procedure .
if weed was really 20% thc then the trichome would have to be 100% thc .
if you extracted the trichomes of avg weed you would be lucky to get 15% trichomes back and you can't have more thc than the gland that makes it .
AMD if you separated it, the resulting resin would have to be 100% thc and be completely free of terpenes and other compounds.
this is a quote from a testing procedure :
Below the HPLC chromatograph are two charts that highlight the cannabinoid potencies of your product. The first chart identifies cannabinoid concentrations (Fig. 3). As you can see, the delta-9-tetrahydrocannabinol (THC) level is 24.64 per cent (the product sample analyzed in this example is a cannabinoid extract, so the concentrations are much higher than what would occur in dried flowers).
I will post sample prep procedures in pics