Il be using tissue culture tek on old seeds and I'm attempting to do this too
The tissue sample is trimmed and goes through a crucial sterilization process. Once cleaned, the plant tissue goes in a dense nutrient culture which is typically an agar gel containing a carefully crafted nutrient, hormone and sugar mixture. The tissue culture controls the sample. Different hormone mixtures will trigger various stages of development for the tiny culture.
The culture can technically remain in a period of indefinite purgatory, until a time when the grower wishes to work with the sample again. Through the introduction of new hormones and nutrient-rich growing solutions, a cultivator can trigger growth, root development, and multiplication. When the plant is large enough to multiply, it is ready to be trimmed into literally hundreds of separate yet precisely cloned individuals.
These hundreds of samples follow a similar course until they are large enough to enter into a phase of hardening off and ready for planting. From one small tissue sample, a well-organized cultivator can potentially create hundreds of perfect clones, without any contamination from the mother.