Care to explain what a double haploid is?
doubling of chromosomes in a haploid cell makes a double hap.
A way to strongly influence probability in our favor...
The ability to make a homozygous line in one round can literally save a breeder a lifetime of work.
Traditional breeding methods are slow, one could fight heterozygosity for years trying to develop a strain. the big deal is more elite can be created and selected within less time.
I am eyeing a professional flow hood, I made one but it sucks ass ha!
doubling of chromosomes in a haploid cell makes a double hap.
A way to strongly influence probability in our favor...
The ability to make a homozygous line in one round can literally save a breeder a lifetime of work.
Traditional breeding methods are slow, one could fight heterozygosity for years trying to develop a strain. the big deal is more elite can be created and selected within less time.
Interesting subject!
Not sure how good that strategy will pay off with cannabis, though. There might be simple protocols which 'accidentally' work on cannabis too but what do you do with a doubled haploid then? It will most likely be a fragile, sick, weak, crippled runt whose only sense in life is being a homozygous carrier of a valuable and/or new mutation which will only come into play once outcrossed. To me right now (I admit, it's been a long day for me) it seems less trouble (and certainly more fun) to create a doubled haploid just because you can than all the crossing with other doubled haploids needed to find the real goal, a 'perfect' F1. Without genetic markers, the value of a DH especially in outcrossing species seems rather low to me.
I was reading into that subject a while back and there's a bunch of feasible strategies for certain crops. Most often, it's a freak of nature or a coincidence which creates and/or rescues the haploids of a given plant species (for example wide crossing in barley or tobacco: doesn't work so far with cannabis x hops, or does it?). Colchicine and IIRC also caffeine are used in the second step where you double the chromosomes. You should probably focus on gyno- and especially androgenesis . If I can re-find those papers again, I'll let you know.
The term "true breeding" simply don't exist in my native language, word for word it mean nothing for me. That's why. It was a good opportunity to decypher it one time for good, i don't have seen a single entry in wikipedia on this exact term. I thinked initially that "true breeding" mean just a good work. But this subject changed this point of view. Thanks for the explanation.
I'm losted in one point. If you consider that "true breeding" equal genetical clones with DH specimens, i don't understand the use of this term also for an (good) inbred line wich is not a genetical clones group.