How long can you keep pollen alive and still get pollination?
How did you keep the pollen?
-SamS
How did you keep the pollen?
-SamS
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HOW OLD CAN POLLEN BE ALIVE?
- Thread starter Sam_Skunkman
- Start date
hi sam, i keep mine in ''00'' gel caps that i get from the chemist. i keep them in the fridge. the gel cap takes the moisture out of the pollen and than the fridge takes it out of the gel cap. i have had pollen that is still viable after two years. only take it out of the fridge when you need it though or else it will die.
I have some Original Haze pollen that is 17 years old, collected, cleaned, dried over night, placed in a paper bindle's, and frozen in glass jars for 17 years.
When I used it recently I got normal seed set.
I have many, many, liters of pollen frozen, the oldest around 20+ years.
-SamS
When I used it recently I got normal seed set.
I have many, many, liters of pollen frozen, the oldest around 20+ years.
-SamS
5 days hanging around in the Postal system in warm Spain seemed to do no harm, kept in a Rizzla paper in a fridge with Silica desiccant for months before and after.
17 years thats awesome
do you keep any kind of desiccant in the jars?
peace,
Infi
mixed with flour in a baby food jar in a box room temp possibly warmer for 4 years now. still viable.
If I may say, i'm very septic about pollen being still viable after more than 3-4 years especially with the storage described by each of you. Pollen viability of the vast majority of angiospermes decrease to arround 10% a few hours after stamen opening. With adequate storage, you may be able to keep it a few years, no more.
did anybody made it germinate on water-sucrose agar and check the emergence of germtubes with microscope ? be careful because the seeds may be parthenocarpic , the developement being triggered by contact between the dead pollen and the pistil and/or aborted germination of non viable pollen.
the best way is still to keep the pollen in vacuum sealed vials at -18°C (see post i did a few month ago on mrnice forum)
http://www.mrnice.nl/forum/6-breede...9520-question-best-way-store-male-pollen.html
good luck
did anybody made it germinate on water-sucrose agar and check the emergence of germtubes with microscope ? be careful because the seeds may be parthenocarpic , the developement being triggered by contact between the dead pollen and the pistil and/or aborted germination of non viable pollen.
the best way is still to keep the pollen in vacuum sealed vials at -18°C (see post i did a few month ago on mrnice forum)
http://www.mrnice.nl/forum/6-breede...9520-question-best-way-store-male-pollen.html
good luck
im not a lab assistant im just telling you what i did and that it still pollinated my plant and more branches than i intended.
it made seeds. experiment done.
it made seeds. experiment done.
ive found that mine (properly dried) only lasts for a year or so in a regular 'frost free' freezer. frost free freezers partially defrost to stop the ice build up so this probably compromises the storage somewhat.
VG
VG
1
187020
Was the male spicy/incense smell? Too much bland sweet haze these days it seems... Nevilles haze still got the church (chaco's 130day pheno)
Peace babeh
he was probably referring to sam, and i can't validate any of his info... but it sounds geeky enough...lol
yeah i think he's saying some percentage of those seeds won't germ and be healthy... so i think his point is this method shouldn't be used to produce seeds for market... but i don't see why it couldn't be used to preserve the genetic make up of a male that is no longer alive as some of the seeds must be viably healthy.
your experiment should
A) never be done
B) at least include growing your seeds to see if you made improvements or detriments to the genome
making seeds with no concern wether they germ and grow quality herb or not is somewhat akin to masturbation.....
sure you probably enjoyed yourself in the process but as far as reproduction what did you accomplish?
1. i never got to see the results.
2.) the pollen is gone in the great pollen losing incident of 2012.
3.) i was trying to accomplish back crossing my strain back to the original male, but now all of that is gone... sigh.
can only incross the offspring now.
people say a lot of shit and claim that it is a rule...then someone proves them wrong... then they argue that they couldn't possibly be wrong. then there is a new rule.
2.) the pollen is gone in the great pollen losing incident of 2012.
3.) i was trying to accomplish back crossing my strain back to the original male, but now all of that is gone... sigh.
can only incross the offspring now.
people say a lot of shit and claim that it is a rule...then someone proves them wrong... then they argue that they couldn't possibly be wrong. then there is a new rule.
that sucks you lost the male.. and its pollen! but did you grow the seeds you made with the 4 year old pollen? you could always try and start over with one of them
seeds never got done, and aside from some residue left on the inside of one of my drawers (which i just scraped up with ear swabs) the male and the pollen are long gone. had a run in with curtis jackson couple weeks back. im hoping the pollen will still be working if i dust some girls with it in the future. if not. still got the offspring
I dried my Pollen on paper for a day and then made single use packs. I put those in a plastic case a sealed it. They are now stored in the freezer.. Its been about 4 months. I have not tried any since I froze it. I hope it stores good. I will be using it again..
Tetrazolium is what I use for testing pollen viability, I find it better then "water-sucrose agar and check the emergence of germtubes with microscope"
And I do agree that the % of frozen viable pollen is only 5-10% or less at the most, but that is enough when you have lots frozen.
I have used lots of pollen that was over 10 years frozen, no problem.
I have not had success with pollen stored at room temps for longer then a year.
I never tried vacuum as I did not seem to need it, so I can not comment.
I freeze mine at -30C in a freezer that does not defrost.
And I use it as fast as I can when removed from the freezer, I am afraid it loses viability quickly. I have used it still frozen when I pollinated large amounts of plants, worked great.
-SamS
bonbolos, how long have you kept Cannabis pollen viable, and used it to grow healthy seeds and plants? What was your technique?
-SamS
And I do agree that the % of frozen viable pollen is only 5-10% or less at the most, but that is enough when you have lots frozen.
I have used lots of pollen that was over 10 years frozen, no problem.
I have not had success with pollen stored at room temps for longer then a year.
I never tried vacuum as I did not seem to need it, so I can not comment.
I freeze mine at -30C in a freezer that does not defrost.
And I use it as fast as I can when removed from the freezer, I am afraid it loses viability quickly. I have used it still frozen when I pollinated large amounts of plants, worked great.
-SamS
bonbolos, how long have you kept Cannabis pollen viable, and used it to grow healthy seeds and plants? What was your technique?
-SamS
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Thanks for that info Sam it came at the perfect time. Aloha
