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Old 06-30-2018, 09:25 PM #41
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"You have to NEVER let the Tissue be EVEN FLASHED FOR 1,000,000TH OF A SECOND TO ANY uva/uvb OR THEY ARE INSTANTLY DEAD.
They are propogated under Cool White Fluoro, and then transitioned over to a Mix of Coll/Warm."

fluors have fair amount of uv?
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Old 07-01-2018, 03:01 AM #42
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Exactly my thought on the subject.
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Old 07-03-2018, 02:10 AM #43
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think he probably heard they need to be hardened off before planting under sun or more then floro power.

I dont see greenhouses setting up TC labs as cannabis is so easily propogated by cutting it really wouldnt save time or space. callus tissue first needs to grow leaves and stem which takes several weeks if not longer then needs to be transfered to gorw roots for week or 2 at which point it needs to be slowly aclimatized or hardened off before planting and will be size of week or 2 old seedling ie few inches tall. while for orchids or strawberries (that need to grow runners to be multiplied) it does make sense I dunno if its economically viable for day to day greenhouse use. you can take cuttings of cuttings in that time with marginally more room and power(taking into account autoclaving energy etc). I dont get that 5-7 years they say as its exponential even starting with 1 large plant

great to clean up plants or store plants as sam mentioned, or for sending var to many greenhouses from one breeder nursery type scenario. but other then that and research I dont seeing it becoming the standard. but if you are a nursery selling plants and want to have thousands of elite var in storage incase someone wants to order 500 mother clones it would be def very useful.
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Old 07-03-2018, 03:56 AM #44
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Maybe clones guaranteed free of problems will become more popular in places. Anyone with mycology gear in the cupboard can spend another $100 and take this out for a spin.
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Old 07-11-2018, 01:35 AM #45
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Proper Sterilization should be one of the first orders of business for proper TC.

I saw the cloudy jar and aluminum foil ..
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Old 07-11-2018, 02:22 AM #46
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Slowly getting up in running thinking the issue I'm gonna run into is the agar.

Go to the Asian grocery
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Old 07-16-2018, 02:08 AM #47
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alum foil is often used in autoclavs pressure cookers as it withstands heat and keeps condensation from entering your jars and keeping a sterile area if change in temp causes any suction as it cools.

If you have ever been in labs you often see aluminum foil on half million dollar machines for insulation.

agar ms salts hormones create foggy look sometimes when its thick, thin layers in petri dishes perhaps are what u have seen and look clearer? charcoal is added and it is BLACK in that case. Agar, if contamed, will grow on surface before inside and def wont grow plantlets.
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Old 07-16-2018, 02:35 AM #48
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alum foil is often used in autoclavs pressure cookers as it withstands heat and keeps condensation from entering your jars and keeping a sterile area if change in temp causes any suction as it cools.

If you have ever been in labs you often see aluminum foil on half million dollar machines for insulation.

agar ms salts hormones create foggy look sometimes when its thick, thin layers in petri dishes perhaps are what u have seen and look clearer? charcoal is added and it is BLACK in that case. Agar, if contamed, will grow on surface before inside and def wont grow plantlets.
After working in a surgical sterile processing unit with commercial autoclaves cooking thousands of instruments a day I can attest we never used aluminum foil or jars.

What a joke.

Oh and also let's not get started on the "if you have ever been in a lab.. we don't wear gloves and use duct tape to hold the aluminum foil down.." Come on, seriously? Why defend BS?
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Old 07-16-2018, 04:33 AM #49
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When I did it back in 95, with a kit from Worms Way, an Aquarium/home made box to work in ect. No carpet, no curtains in the bathroom, completely sterilized with Lysol, and also spraying the air to keep down dust.
You shouldn't have any kind of cloth, carpet, curtain type materials within 500 miles of the operation. That shit = dust/mites/mold. All 3. All surfaces completely wiped down. I put Sarah Wrap all over myself, and sprayed it down with Lysol. DRIPPING ect, and every step you were constantly spraying down with Lysol.( Don't forget to sterilize the sprayer. ) If you blinked your eyes, you sprayed… joking of course, but its all about sterile, nothing floating in the air, using an enclosed box ect, sterilized, if 1 does use use aluminum foil, Im sure its sterile. I wrapped myself in Saran Wrap , and sterilized it before wrapping, and after.

If ANYTHING, more simple than the plant tissue gets in the Agar/Medium. Its done.

Also the UV coming out of a Fluro, is nothing, as the glass takes out any UV that may be produced.

You have to have really, really good glass for UVA/UVB Bulbs.
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Old 07-16-2018, 09:12 PM #50
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https://3c1703fe8d.site.internapcdn....aystudypro.jpg

All i am saying is what u pointed out as bas is actually pretty common. also jars are very common in mycology(canning jars) or tissue culture (esp old used baby food jars ). So dont jump up peoples asses when u do not know whats actually going on "omg what a jerk doesnt have a laminar flow hood"
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