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Old 07-16-2018, 09:52 PM #51
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Old 10-02-2018, 08:30 AM #52
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how fragile are these smalll tubes of tissueculture to temperature swings and lightloss for longer periods, is it the same as when mailing clones or are they more fagile
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Old 10-02-2018, 01:15 PM #53
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Old 10-03-2018, 10:12 AM #54
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Quote:
Originally Posted by CFP65 View Post
how fragile are these smalll tubes of tissueculture to temperature swings and lightloss for longer periods, is it the same as when mailing clones or are they more fagile
you likely would receive as a rooting plantlet, which will be fine as far as light can always add small watch battery and led. they are ran on hormones and sugars and salts so use that more then producing it own energy.

they are pretty fragile once out of the tube and need to be kept humid and slowly introduced to unfiltered light as leaves are paper thin and used to basically a womb if you will. perhaps would ship at normal clone size already hardened off which of course takes extra care time and space which sort of defeats the purpose of microcultures small and easy to ship format.

guys in colorado tried this already and hasnt worked out. but is great for archiving cultivars. in my limited experience with tissue culture its not as fast as taking a clone rooting it and taking clones of those clones. renews and cleans old clones tho so def has a place. I think there will be a huge hord of genetics by LPs not wanting to share there proprietery var.
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Old 10-04-2018, 08:43 AM #55
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I have seen references to tissue culture ever since I started coming here. 10 years ago there was a lot of activity on the subject but then it kinda died down. What I remember reading in articles was that cannabis was significantly more difficult to work with than strawberries.
I don't think we will be seeing a lot of tissue culture happening on the hobbyist level, although whereever weed is legalized I wouldn't be surprised to see clones from tissue culture being offered - perhaps on demand.
What is the timeframe from starting a tissue culture to a clone in growing media (rockwool, soil etc)?
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Old 10-06-2018, 09:47 AM #56
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45 days ish ruff estimate if starting from calus then few weeks to grow a leaf/stem few more to get roots and u have a 2 inch plantlet that needs to be hardened off for 5 days. so couple months i guess. email bubblemans buddies company and see what they say and get back to us.

wish them all the luck but other then storage of genetics and maybe clean up a cut or 2 cant see it being economical as normal cuts are so easy cheap and much larger then microculture technics.

One glaring issue which hasnt been brought up yet is the chemicals used 1 100% PGR loaded, plant steroids to allow speedy growth, also toxic antibiotic antifungals used to keep everything clean. cant imagine it passing the tests. hope that wasnt a huge oversite!! also dont want them petitioning/lobbing HC to allow the "normal" agriculture chemicals!!!

they have been purchased by largish LP supreme so bet they will spend the cash to allow the PGRs which is unfortunate. the issue arrises as health canada may have hard time admitting these PGRs are harmful as perhaps allowed in strawberry plants. but i think they have realized inhalation is different then edible(even when edible is "bad"). guess time will tell and PGRs are a huge no no in legal states so imagine we will adobt such a standard. anyhoo love to hear the reply about PGRs from the company.. tuff crowd to say oh dont worry small quantitys.....

Last edited by clearheaded; 10-07-2018 at 08:22 AM..
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Old 10-07-2018, 08:26 AM #57
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Originally Posted by marmarb View Post
Slowly getting up in running thinking the issue I'm gonna run into is the agar.
agar is no issue and MS salts are relatively cheap. google MS salts will help u get a handle on what is required.
as someone said bill graham is a HUGE resource and will help you out for a home kit
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Old 10-10-2018, 08:50 AM #58
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bubbleman got the DNA boys in, first to market i guess is neat? wise investment sees anyone can do it inexpensively.

but again id really love to hear how they produce plants that can pass for PGR and cancer causing antifungals antibacterials used. one of those things thats different from edible world and cannabis..

Last edited by clearheaded; 10-11-2018 at 07:21 AM..
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