Originally Posted by led05
You brothers are amazing, what you're doing great but this post in a way strikes me as arrogant, unlike any other you've ever done...
don't get too big for them britches and remember your roots
Thanks for your work
if I had to guess, offers or $$ flow is a coming, stay true
Well shit, no arrogance intended and apologies to everyone if it came across that way. Thanks for the kind words and redirect if I was out of line. Sam has always provided the best advice in terms of searching for something unique (grow as many plants as possible), so the end of the post was supposed to reflect that sage, but often overlooked wisdom.
I thought Mate Dave was asking about the youPCR picture that Sam posted and how that would / could translate to breeding. We've been working with the platform since last summer. Screening for male / female is robust, accurate, fast, relatively cheap. Screening for active CBDa or THCa alleles is much more difficult. Kevin McKernan @ Medicinal Genomics is always working to make the test better by adding new active allele data as it comes in, but the test is very temperature sensitive and we are still getting occasional false positives on the CBDa side. We use the youPCR screen to segregate type I / type IV and type III / type IV in our current projects.
The platform works great for type I / II / III segregation that many people are working on to breed better type IIs and IIIs. When a pure THC parent (P1) is crossed with a pure CBD parent (P2) and the resulting progeny open pollinated / selfed, the square for F2s looks like this (generated from the previously linked website) :
T = functional THCa allele
t = nonfunctional THCa allele
D = fuctional CBDa allele
d = nonfunctional CBDa allele