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cannabis to form callus in micropropagation medium

strangladesh

New member
will 6-bap work to form callus growth. Can someone give me a break down of a good process to tissue culture mj? Are there any other hormones that i will need? Any help would be great thanks.
 

Sokan

Member
ive tried MS(salts and vitamins) +1ml PPM+1ml NAA + 1ml kinetin - thought it will work nice on forming callus...and it did but unfortunately just after callus formed, lots of roots started to grow, so for next run I decided to use 1ml NAA and 0 of kinetin to form some shoots...do not have any results yet :)
still, the biggest problem that i had working on tc was sterilization.:) and not only when you transfer explant to medium tubes, but after that..you have to keep all the tubes in clean environment otherwise all the samples will be contaminated with a fungi or bacteria or both.
 
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