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Old 04-20-2017, 08:18 PM #321
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I do agree that environmental influences can change the ratios, I have seen the same clone do this all dependent on the environment. But to see a major terpene appear or disappear is something I have not seen like this example. I am suspect to say the least. I have seen some of the major testing labs get results that was not correct, I know because the identical clone sample was sent to half a dozen labs and they were not all the same results, some identified peaks as the wrong terpene, as well some are using HPLC to test for terpenes when all the major terpene labs use GC-FID or some GC-MS. As well some labs test time for runs is much faster then we used, we wanted clear separation between peaks we wanted the best results we could get, they are driven by maximizing the amount of samples that can be run in a day. You decide what you would want.
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Originally Posted by Sativied View Post
That appears to be greengenes' test result. No controls, no consistency in growing, no repeat results, could be a lot of other things. On the other hand, I would not be surprised at all if environmental influences can change the ratios to a point where one is not detected. Unless you can reproduce the results it's rather meaningless.

There is plenty of research that shows certain light recipes and intensity as well as nutrient availability heavily influence secondary metabolites production.
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Old 04-21-2017, 01:53 AM #322
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Sam_S, Sativied, thank you for setting this straight.

Yep this test picture is from greengenes.

So Sam_S, if there is any particular way to increase overall terpene content? The way i interpret your previous post is that the terpene content is genetically determined, is that correct?

Let me use this example. These are the tests of the same hybrid coming from the same dispensary. I don't know if they run the same clone. It is my assumption that they may do, because the terpene ratios look almost identical. But terpene content is drastically different on all 3 samples. That draw me to the question if some environmental factor played a role here? Is there a way to boost terpenes' content from 2% to 5%?

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Old 04-21-2017, 02:34 AM #323
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Originally Posted by Gr33nSanta View Post
I own some 3000k, 3500k and 4000k, I like to mix em all, I also have a few old blurples in the mix. It is said that the red increase yield while the blue to some extent increase resin production.

Since I am trying to grow resin I never even tried growing with the 3000k alone. I get great results from the 3500k by themselves or mixing the 3000k and the 4000k. I have also seen great results mixing 4000k and 5000k in flowering but more testing is required.

most leds I use are cxb3590, vero 29 and also I have some other older and newer tech. I mix em all, 100% LEDs in flower, since 2011


interesting that man has made such an impact on all cannabis due to the GREED of products that could be replaced by hemp and sativa l


when america goes fully legal then we will see the bio agro companies really impact cultivars and landrace cannabis.

as far as light impact studies determining profile in genetic and genotype regulation of chemical production there are studies but with money possibly tainting outcomes we need verified testing through unbiased sources.
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Old 04-22-2017, 02:21 PM #324
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Originally Posted by Nirrity View Post
Sam_S, Sativied, thank you for setting this straight.

Yep this test picture is from greengenes.

So Sam_S, if there is any particular way to increase overall terpene content? The way i interpret your previous post is that the terpene content is genetically determined, is that correct?

Let me use this example. These are the tests of the same hybrid coming from the same dispensary. I don't know if they run the same clone. It is my assumption that they may do, because the terpene ratios look almost identical. But terpene content is drastically different on all 3 samples. That draw me to the question if some environmental factor played a role here? Is there a way to boost terpenes' content from 2% to 5%?

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using more light and using bug dunks (plants think bugs are attacking it) maybe.
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Old 04-22-2017, 04:12 PM #325
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interesting that man has made such an impact on all cannabis due to the GREED of products that could be replaced by hemp and sativa l


when america goes fully legal then we will see the bio agro companies really impact cultivars and landrace cannabis.

as far as light impact studies determining profile in genetic and genotype regulation of chemical production there are studies but with money possibly tainting outcomes we need verified testing through unbiased sources.
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Old 04-22-2017, 04:50 PM #326
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Old 05-07-2017, 02:49 PM #327
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Sam, you call CBD the anti-weed (or something similar) , like CBD is your biggest enemy. But what do you think of using CBD in lieu of tolerance break? I find if I smoke high CBD cultivars for a couple of days in a row, and then smoke a high THC cultivar, I get so so high from it.
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Old 05-07-2017, 03:02 PM #328
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That makes sense to me, but what I smoke is so strong I do not have problem with tolerance I assure you I can easily get medicated as a kite everyday with no problem. If you find it handy then use it. I have no need to adjust my tolerance, as long as I have great resin. I think you may know I do not smoke herbal Cannabis.
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Originally Posted by Gr33nSanta View Post
Sam, you call CBD the anti-weed (or something similar) , like CBD is your biggest enemy. But what do you think of using CBD in lieu of tolerance break? I find if I smoke high CBD cultivars for a couple of days in a row, and then smoke a high THC cultivar, I get so so high from it.
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Old 05-07-2017, 04:31 PM #329
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can you name a few cultivars that produce your great resin? I am sure you have access to the best of the best but which ones do you rarely go a week without smoking? any of your old varieties still in rotation in your own garden?
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Old 05-07-2017, 05:30 PM #330
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Originally Posted by Sam_Skunkman View Post
https://journals.plos.org/plosone/art...l.pone.0173911

Terpene synthases from Cannabis sativa
Judith K. Booth, Jonathan E. Page, Jörg Bohlmann
Published: March 29, 2017https://dx.doi.org/10.1371/journal.pone.017391



I liked this paper because I bred FINOLA, will be interesting when they do the same with more Cannabis varieties that yield closer to 10% terpenes, instead of the normal 1-2% terpenes found in most. They also show work with Skunk #1 and Purple Kush, I did not breed Purple Kush, I think I did all the others. Breeding for terpene profiles and %'s will be the future, work like this a very small step in that direction.
-SamS
That is cool- and further I'm glad that we're getting more information on so many more secondary pathways and isoprenylation in cannabis specifically. Identifying each pathway and their corresponding terpene expression is huge.

"The present study highlights the large number of CsTPS genes and the diverse products of the encoded TPS enzyme activities, which contribute to the complex terpene profiles of cannabis. The knowledge of multigene nature of the CsTPS family and the often multiple products of the encoded enzymes will be critical when selecting or breeding, or improving plants by genome editing, for particular terpene profiles for standardized cannabis varieties. While cannabinoid-free individuals have occasionally been reported [48], we are not aware of any reports in the literature of terpene-free cannabis. In this study, we observed a single monoterpene-free individual, which however still contained cannabinoids and sesquiterpenes. This observation implies that biosynthesis of the different classes of terpenoid metabolites are independently regulated."
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