[Palindrome]

Impressive work WaterFarmFan

30-100k seeds of 280 som crosses, gonna be an impressive collection.
Just the thourt of sorting out all thouse seeds, is head spinning. Once done, I wouldn't know where to start.

Looking forward to see, where you go from there.

[WaterFarmFan]

Thanks bud. This has been a lot of work at every step. I spent ~25 hours just doing the hand pollination in three stages, as I staggered the starting of the females. Sorting the seeds will be done over a few weeks, so it should not be too bad. By the time the females finish, I should have a good idea idea on which lines that I want to start exploring.

[WaterFarmFan]

Here is the final lineup that I used to make crosses. I dropped 4 females and 3 males that I did not think were up to par.

Females

Ace - Malawi (Fem) #2 _ Medium racy pheno
Ace - Malawi x Pakistani Chitral Kush #4 _ Purple frosted spear pheno
Ace - Malawi x Panama #2 _ Massive cola 50/50 pheno
Ace - Panama #1 _ Chunky inbred pheno
Ace - Panama #5 _ Goddess pheno
Ace - Panama #7 _ Red pheno
Ace - Zamaldelica (Fem) #1 _ Tall Zamal/Thai pheno
Bodhi - Goji OG (Nepali OG x Snow Lotus) #2 _ Chunky snow lotus pheno
Bodhi - Goji OG #7 _ Berry frost elite pheno
Bodhi - Space Cake (Forum x Snow Lotus) #2 _ Forum cookie pheno
Bodhi - Space Cake #9 _ Thin, dense PB cookie
Bodhi - Space Monkey (GG4 x Wookie #15) #2 _ Huge bush glue leaner
Bodhi - Space Monkey #3 _ Medium 50/50 pheno
Bodhi - Sunshine Daydream (Bubbashine x Appalachia) #2 _ Butter biscuit pheno
BOG - SSK (Strawberry Cough x Razzberry Kush x ECSD) #1 _ Strawberry power pheno
Cannarado - Sundae Driver (Fruity Pebbles x Grape Pie) #4 _ Sweet grape jelly pheno
Dying Breed - Watermelon Zum Zum #9 _ Fat melon pheno
Exotic - Thug Life (Extreme OG X Triple OG) #6 _ Pale frost pheno
In House - Gorilla Dosha (GG4 x Dosidos) #4 _ Glue leaning pheno
In House - Gorilla Dosha #5 _ Glue dom pheno
In House - Gorilla Dosha #6 _ Dosidos dom pheno
Karma - Dominator (Kushage x Jack Herrer) #2 _ Spicy Jack pheno
Karma - Road Dawg (Stardawg x Biker Kush) #5 _ Stardawg chemy funk pheno
Thug Pug - Cactus Breath (Cactus x Mendo Breath) #3 _ Frosty stout branchy pheno
Tony Green - Gorilla Bubble Bx3 #4 _ Squat SB leaning pheno
Top Dawg - Chem D x i95 F2 #3 _ Chem D leaning pheno
WFF - Double Strawberry Diesel F2 (ft100) x Sour Lifesaver #3 _ Golf ball fruity fuel pheno

Males

Ace - Panama #6 _ Red/Goddess pheno
Ace - Panama #8 _ Chunky inbred pheno
Ace - Zamaldelica #4 _ Squat Malawi frosty pheno
Ace - Zamaldelica #6 _ Tall Zamal/Thai pheno
DVG - Citrus Farmer (Skunk Tangerine x Grandpas Breath) F2 #2 _ Citrus Breath pheno
DVG - Citrus Farmer F2 #7 _ Chunky grapefruit pheno
DVG - False Teeth (Candyland x Grandpa's Breath) #1 _ Pure Breath pheno
DVG - Foul Mouth (Dookies x Grandpas Breath) #6 _ Strechy Cookie pheno
Dying Breed - Watermelon Zum Zum #1 _ TBD
Dying Breed - Watermelon Zum Zum #4 _ TBD
Dying Breed - Watermelon Zum Zum #8 _ TBD
Karma - Road Dawg #4 _ Bushy medium strech pheno
Med Tree - Richness (ACDC x Rainmaker) #5 _ 20:1 High CBD pheno
Thug Pug - Cactus Breath #1 _ Tall beefy pheno
Thug Pug - Child's Breath ((Cherry Puff x Grape Stomper OG) x Mendo Breath) #7 _ Cherry Pie structure pheno
Thug Pug - Meat Madness (Meatloaf X (Mendo Purps x Crystal Locomotive F3)) #2 _ Huge vigorus pheno
Thug Pug - Peanut Butter Breath (Dosidos x Mendo Breath) #3 _ Pure Breath pheno
Thug Pug - Grape Breath (Grape Stomper x Mendo Breath) #1 _ Pure Breath pheno
Tony Green - Gorilla Bubble bx3 #2 _ Stretchy glue pheno
Top Dawg - Chem D x i95 F1 #1 _ TBD
Top Dawg - Chem D x i95 F1 #4 _ TBD

[Gr33nSanta]

wait, is that 27 x 21 = 567 new cultivars? you are going to need an army of helplers and many acres to sift through that stuff!!

[WaterFarmFan]

Originally Posted by Gr33nSanta

wait, is that 27 x 21 = 567 new cultivars? you are going to need an army of helplers and many acres to sift through that stuff!!

Hehe This is a long-term project for me, but I just might get some testers out to share some love and see what these can do in a shorter time frame. If seedbay got it's act together, I would donate multiple lots of several dozen strains...

There are a total of 567 combos potentially at play with cross-contamination, but each female plant had a different number of tops and thus a different amount of branches to dust. Some would get 8 others 15, but the average was just over 10 crosses per female (~280). I really took my time hand dusting each flower node, and covered each quite extensively, so I think that cross-contamination will be relatively low, but might occur a bit later as new stigma form. Pollen dust was flying and some likely landed on unintended places, but I think enough landed in the right places to make the contamination statistically smaller, which I hope would be in 10 percent range but could be closer to 20. If I grow out enough of these seeds from the same mom and different males, I should be able to spot similarities and differences.

Here was my basic pollination process:

In a large still room, I arranged 9 females in a semicircle with several feet between plants. I then pulled a packet of pollen for one male from fridge and marked it on my master list. Each male got a number. I then decided which females that I wanted to hit with that male, and made a corresponding number of labels. I then selected branches and applied labels all at once. With a clean paint brush (available cheap by the dozen on Amazon), I then dusted all labeled branches for crosses that wanted to make with that one male. I would place brush in bucket to later clean with acetone before next use.

I repeated this process for each male (21 total) that I collected pollen from. Some males hit almost every female while others were quite less. For strains that had multiple males (Panama, Zamaldelica & Zum Zum), I would often combine the pheno pollen packs into one, mix thoroughly and apply as a single branch to get max genetic diversity. These multiple pheno mixes got a number as well.

After all 9 females were completely dusted and labeled, I placed in a relatively still 5x5 tent for a couple of days to isolate, and then raised humidity (humidifier) over night to denature pollen before putting in main room. I did this process 3 times over 2 1/2 weeks in groups of 9 females.

While I had a short list of desired crosses for each female, I really had to think about traits as there were limited slots. I don't know what you call it, but I certainly felt like a real breeder...

WFF

[WaterFarmFan]

And just for the record, I know that this is a big ass, albeit as highly controlled as possible, pollen chuck, but this method was the only way that I could come up with to make the most seeds, with the most genetic diversity and in the shortest amount of time. The real breeding will begin when I begin to hunt the lines and make F2s, F3s and beyond to stabilize traits.

I have a dream, and it involves HPLC (cannabinoids) & GC-FID (terpenes) onsite testing of large numbers of these cultivars...

[WaterFarmFan]

Looking back, and seeing now how crazy all of this really was, I started this whole project with the simple goal of combining a few sativas with a couple of beefy high-thc indicas to make some beans. Then I just kept buying seeds and finding new awesome plants that I wanted to cross and before I knew it, here we are...