[PhenoMenal]

A = CBD Therapy - Veg phase, 40 days from seed
B = Dinamed - Veg phase, 24 days from seed
C = Dinamed - 2wks into flower phase
D = Dinamed - 4.5wks into flower phase
E = CBD Therapy - harvested 55 days into flower
F = Dinamed - harvested 61 days into flower

Only lanes A and E are showing CBD (the large orange dot above the red dot near the top of the plate)

The Dinamed CBD was a disappointing result -- no CBD at all from this particular phenotype. The first Dinamed i grew was 1:1.

[dybert]

Originally Posted by PhenoMenal

View Image
A = CBD Therapy - Veg phase, 40 days from seed
B = Dinamed - Veg phase, 24 days from seed
C = Dinamed - 2wks into flower phase
D = Dinamed - 4.5wks into flower phase
E = CBD Therapy - harvested 55 days into flower
F = Dinamed - harvested 61 days into flower

Only lanes A and E are showing CBD (the large orange dot above the red dot near the top of the plate)

The Dinamed CBD was a disappointing result -- no CBD at all from this particular phenotype. The first Dinamed i grew was 1:1.

Nice separation!

[PhenoMenal]

cheers, yeah i usually get excellent separation from chloroform, as you've also personally experienced! ... its just a bit less hassle than measuring out the 20:80 diethyl ether : hexane (and chloroform doesn't assault the nostrils as bad as diethyl ether, that stuff is hardcore!), but as i also use hexane for the extraction phase i'm starting to run low on hexane!

so now I usually just do chloroform separations, but when I do some seed runs soon (~12-16 at a time, then destroy all but the two with the best CBD profiles after ~3 weeks) I'll do just chloroform initially, but then use both methods on the two 'winners', as it gives a slightly different 'viewing angle':

Apparently it will be even better separation if i let the plate fully dry and then re-do the mobile phase - it should use most of the plate then (no need to do that with chloroform though which already uses the whole plate). Hope to try the 'double-dip' soon.

It's weird how the shape of the base of the jar (eg having more eluent depth around the edge than the middle of the jar) effects the shape of the TLC with a slight curve of the results ... or at least, it does with chloroform, but that doesn't happen with hexane : diethyl ether. *shrug!*

[dybert]

Originally Posted by PhenoMenal

cheers, yeah i usually get excellent separation from chloroform, as you've also personally experienced! ... its just a bit less hassle than measuring out the 20:80 diethyl ether : hexane (and chloroform doesn't assault the nostrils as bad as diethyl ether, that stuff is hardcore!), but as i also use hexane for the extraction phase i'm starting to run low on hexane!

so now I usually just do chloroform separations, but when I do some seed runs soon (~12-16 at a time, then destroy all but the two with the best CBD profiles after ~3 weeks) I'll do just chloroform initially, but then use both methods on the two 'winners', as it gives a slightly different 'viewing angle':
View Image
Apparently it will be even better separation if i let the plate fully dry and then re-do the mobile phase - it should use most of the plate then (no need to do that with chloroform though which already uses the whole plate). Hope to try the 'double-dip' soon.

It's weird how the shape of the base of the jar (eg having more eluent depth around the edge than the middle of the jar) effects the shape of the TLC with a slight curve of the results ... or at least, it does with chloroform, but that doesn't happen with hexane : diethyl ether. *shrug!*

For sure... I always used chloroform for both the extraction and mobile phase.

[PhenoMenal]

ahh, cool! ive only ever tried hexane for the extraction phase... i'll definitely try to remember to try chloroform on the next run, as well as diethyl ether on its own (might as well, i dont use much of it), so i can compare ...

I've done a side-by-side comparison before but that was just of the mobile phase eluent (all extractions were hexane) ...


that's pretty cool though that chloroform can be used for both stages, simplifies things a bit for those wanting to get started with TLC! "just get a bottle of chloroform, some Fast Blue B or BB, some TLC plates, and you're good to go"

[dybert]

Originally Posted by PhenoMenal

ahh, cool! ive only ever tried hexane for the extraction phase... i'll definitely try to remember to try chloroform on the next run, as well as diethyl ether on its own (might as well, i dont use much of it), so i can compare ...

I've done a side-by-side comparison before but that was just of the mobile phase eluent (all extractions were hexane) ...
View Image

that's pretty cool though that chloroform can be used for both stages, simplifies things a bit for those wanting to get started with TLC! "just get a bottle of chloroform, some Fast Blue B or BB, some TLC plates, and you're good to go"

Way simpler, as long as you can get a hold of chloroform!

[PhenoMenal]

Originally Posted by dybert

Way simpler, as long as you can get a hold of chloroform!

yeah, im a bit fuzzy about which chemicals are 'monitored' etc, or if quantities are important etc, and its different in each country, but you should be able to email your local lab supplies company "im after 250ml to 1L of (chloroform/whatever) suitable for Thin Layer Chromatography" ... this way they give you the right grade eg AR/analytical (sometimes they have half a dozen different grades of the one chemical, and I dont know wtf i should be getting!), and it probably sets off less flags saying outright you only want a small amount and it's for a specific scientific use. You might also be able to find them on ebay, and some lab supplies shops sell them online.

Fortunately a small bottle goes a long way with Thin Layer Chromatography! I have my fingers crossed the chloroform will prove to be as good at extraction as hexane when i get to do the side-by-side tests, and your TLC plate a few posts back showed it seems to work just fine