Tissue Culture : Micropropagation - Page 4 - Cannabis Business Network - International Cannagraphic Magazine Forums

ambertrichome · 03-09-2018, 06:19 PM

With TC, you can also culture from culture, and have 0 Genetic Drift. It takes it back to the embryonic stage.

IMHO, like a lot of stuff.

It can be used for good, or bad.

beta · 03-09-2018, 06:41 PM

Quote:

Originally Posted by ambertrichome

With TC, you can also culture from culture, and have 0 Genetic Drift. It takes it back to the embryonic stage.

IMHO, like a lot of stuff.

It can be used for good, or bad.

There is zero evidence that 'genetic drift' is a thing.

Palindrome · 03-11-2018, 01:35 PM

Hello Bubbleman, long time no see.

10 years ago when I had to leave growing, I was looking into TC and in the middle of building a lab. Just for private use, but it have huge potential! As a aquarist, I have for years been buying TC aqua plants. For the very reason, that they are 100% virus and pest free.

Would love to be able to work at home with TC cannabis, maybe have gel cultures shipped over seas.

**guineapig** · 03-18-2018, 01:24 PM

Viruses and bacterial invasion as well when cloning is a less-than-laboratory environment....

Not to get off topic, but does the tobacco industry do this as well, in order to prevent the dreaded Tobacco Mosaic Virus? Hate to bring up tobacco, but the science behind tobacco cultivation might be applicable to the future of cannabis....

kind regards from guineapig

marmarb · 03-18-2018, 03:07 PM

I'm looking at these kits and just going to buy parts separately. Put up a 5x5 tent which I'm going to use my lab zipped up inside air flow would be limited with a diy laminar hood.

Pédetoddy · 03-18-2018, 03:23 PM

Cannabis micropropagation attempt showing fungal and bacterial contamination.

Virgin culture medium

I am experienced in cloning mushroom, have a SAB (Still air box).

beta · 03-18-2018, 06:05 PM

Quote:

Originally Posted by Pédetoddy

Cannabis micropropagation attempt showing fungal and bacterial contamination.
View Image
Virgin culture medium
View Image
I am experienced in cloning mushroom, have a SAB (Still air box).

What was your process for sterilizing the plant matter? How big were your pieces?

Stickybred420 · 03-18-2018, 09:43 PM

https://www.youtube.com/watch?v=bi755vQVNx8

https://www.youtube.com/watch?v=B3yi5U9Cg64

theres some good info here guys! bill graham is pretty legit too.

hamstring · 03-18-2018, 11:01 PM

There is good and bad in all technological advances . There is no free lunch or yin/yang. Pick your proverb.

I am not really commenting on the technology more on the use of it. I am not a huge fan of loosing genetic diversity. The implications of monocropping are well documented.

Yellow dent #2 for instance. It worked so well that they had to build a bunch of fake industries to support the huge crops.

Corn sweeteners have taken over every processed food in the market.

They decided feeding it to cattle would be a good idea. It turns out cows on exclusively corn diets get sick a lot. More antibiotics are used in the commercial meat industries than on humans.

Subsidizing farmers didn't help either but the true explosion of use was because the shit works. Grows fast ,need less water, pest, heat and drought resistance. I'm from the midwest you cant kill ole yeller dent #2.

Just because you can doesn't necessarily mean you should. No free lunch

marmarb · 03-19-2018, 01:10 AM

Slowly getting up in running thinking the issue I'm gonna run into is the agar.

03-09-2018, 06:19 PM	#31
ambertrichome Member Join Date: Mar 2013 Posts: 143	With TC, you can also culture from culture, and have 0 Genetic Drift. It takes it back to the embryonic stage. IMHO, like a lot of stuff. It can be used for good, or bad.
	Quote 1 members found this post helpful.

03-11-2018, 01:35 PM	#33
Palindrome Member Join Date: Jan 2018 Location: Republic State of Banana Posts: 193	Hello Bubbleman, long time no see. 10 years ago when I had to leave growing, I was looking into TC and in the middle of building a lab. Just for private use, but it have huge potential! As a aquarist, I have for years been buying TC aqua plants. For the very reason, that they are 100% virus and pest free. Would love to be able to work at home with TC cannabis, maybe have gel cultures shipped over seas. __________________ A New Start - diary of a retired farmer
	Quote

03-18-2018, 03:07 PM	#35
marmarb Member Join Date: Apr 2010 Location: prop deez Posts: 495	I'm looking at these kits and just going to buy parts separately. Put up a 5x5 tent which I'm going to use my lab zipped up inside air flow would be limited with a diy laminar hood. __________________ Going Vert no Dirt
	Quote

03-18-2018, 11:01 PM	#39
hamstring Senior Member Join Date: Aug 2005 Posts: 1,956	There is good and bad in all technological advances . There is no free lunch or yin/yang. Pick your proverb. I am not really commenting on the technology more on the use of it. I am not a huge fan of loosing genetic diversity. The implications of monocropping are well documented. Yellow dent #2 for instance. It worked so well that they had to build a bunch of fake industries to support the huge crops. Corn sweeteners have taken over every processed food in the market. They decided feeding it to cattle would be a good idea. It turns out cows on exclusively corn diets get sick a lot. More antibiotics are used in the commercial meat industries than on humans. Subsidizing farmers didn't help either but the true explosion of use was because the shit works. Grows fast ,need less water, pest, heat and drought resistance. I'm from the midwest you cant kill ole yeller dent #2. Just because you can doesn't necessarily mean you should. No free lunch
	Quote 1 members found this post helpful.

03-19-2018, 01:10 AM	#40
marmarb Member Join Date: Apr 2010 Location: prop deez Posts: 495	Slowly getting up in running thinking the issue I'm gonna run into is the agar. __________________ Going Vert no Dirt
	Quote

03-18-2018, 01:24 PM	#34
guineapig Moderator Join Date: Aug 2005 Location: In spite of my rage I am still just a rodent in a cage Posts: 4,601	Viruses and bacterial invasion as well when cloning is a less-than-laboratory environment.... Not to get off topic, but does the tobacco industry do this as well, in order to prevent the dreaded Tobacco Mosaic Virus? Hate to bring up tobacco, but the science behind tobacco cultivation might be applicable to the future of cannabis.... kind regards from guineapig
	Quote

03-18-2018, 03:23 PM	#36
Pédetoddy Newbie Join Date: Nov 2017 Location: Lost in savanaah Posts: 45	Cannabis micropropagation attempt showing fungal and bacterial contamination. Virgin culture medium I am experienced in cloning mushroom, have a SAB (Still air box).
	Quote

03-18-2018, 09:43 PM	#38
Stickybred420 Member Join Date: Oct 2017 Location: right behind you. Posts: 253	https://www.youtube.com/watch?v=bi755vQVNx8 https://www.youtube.com/watch?v=B3yi5U9Cg64 theres some good info here guys! bill graham is pretty legit too.
	Quote