Dud Identification Collective Knowledge.

[xxxstr8edgexxx]

What kind of microscope do you have????

nothing fancy at the moment im using a couple dinalites http://www.microscope.com/dino-lite-ad7013mt-50mp-metal-microscope.html

and a cheaper one

http://www.microscope.com/digital-m...o-lite-am3111-0-3mp-digital-microscope-1.html

i would be using someone elses stereo microscope desk compound disecting scope at about 80x for the procedure im talking about above. in a laminar flow hood.

 

[Sam_Skunkman]

do you think microtip culture would be sufficient. or are you really talking single cells. im attempting a cloning this month using the apical dome emerging meristem cells as they emerge but it wont be single cells but rather fresh emerging undifferentiated clusters.

Sounds like you are not using in-vitro?
Rapidly rowing meristems, the smaller of the tip of the meristem as you can, would be less likely to have nematodes, but I have never used this method and am not sure. It is used to eliminate viruses.
I did mean single cells, or small clumps of callus, both of which I have worked with, as well as meristems, and found it fairly easy if you stick to published guidelines and have a HEPA filter with a hooded work bench (laminar flow hood) and a few more needed basics like an autoclave and scale, refrigerator, Various chemicals and sterilizing agents, etc.
The advantage of an invito meristem is they are easy to root, while single cells you get to make callus and while growing callus is easy, and rooting callus is easy, getting callus to make shoots has been very difficult until quite recently.
Good luck,
-SamS

 

[xxxstr8edgexxx]

i am in fact using invitro cloning. root callus? no. meristem invitro cloning. either the macrotip or microtip depending on why im doing it. microtip for clearing virus, macro for general clean genetic storage or transfer.
im very new to it though. im not cryogenically freezing them like you know who or anything. just run of the mill invitro tissue culture. i think it will be a good way to rid the nematodes.
im using a freinds hood at the moment but he has a an extra laminar flow hood filter hes selling me on the cheap. im buiding a 4x2x3 area bench.fingers crosseed. im also looking to build a thermal cycler this winter.

Sounds like you are not using in-vitro?
Rapidly rowing meristems, the smaller of the tip of the meristem as you can, would be less likely to have nematodes, but I have never used this method and am not sure. It is used to eliminate viruses.
I did mean single cells, or small clumps of callus, both of which I have worked with, as well as meristems, and found it fairly easy if you stick to published guidelines and have a HEPA filter with a hooded work bench (laminar flow hood) and a few more needed basics like an autoclave and scale, refrigerator, Various chemicals and sterilizing agents, etc.
The advantage of an invito meristem is they are easy to root, while single cells you get to make callus and while growing callus is easy, and rooting callus is easy, getting callus to make shoots has been very difficult until quite recently.
Good luck,
-SamS

 

[Storm Shadow]

Anyone who wants to do some great reading on our enemy should check out this book.....its a nice boost of knowledge

http://www.dbbe.fcen.uba.ar/contenido/objetos/Agrios2005PARTEI.pdf

PLANT​

PATHOLOGY

 

[mojave green]

agree on that, just so many variables to deal with. Deezzy brought out another good point about the single branch duds, how could they get on one branch and not the whole plant ?

"Many infections caused by fungi, bacteria, nematodes, viruses, and parasitic higher plants are local, i.e., they involve a single cell, a few cells, or a small area of the plant. These infections may remain localized throughout the growing season or they may enlarge slightly or very slowly."
PLANT PATHOLOGY
GEORGE N. AGRIOS
Department of Plant Pathology
University of Florida

 

[Stayfoolish]

We need nano bots to kill terminator nematodes. 15 years away?

 

[RetroGrow]

We need nano bots to kill terminator nematodes. 15 years away?

We already have them.They are called predatory nematodes.

 

[Loc Dog]

Has anyone had this with hydro only grows?????

 

[high life 45]

Yes I was in "hydro" although some might call it soiless, I grew in diatomite and pumice.

 

[xxxstr8edgexxx]

Has anyone had this with hydro only grows?????

my buddies in straight coco hydroton got it.

 

[Loc Dog]

Yes I was in "hydro" although some might call it soiless, I grew in diatomite and pumice.

Was it flood and drain?

That was my favorite, which I used for autoflowers. had total control with central reservoir. Maintain PH, and watch whether PPM went up or down, to know if feeding too much or too little. Found half of GH flora series recommendation was right for 90 day auto flowers.

Did not realize the benefit of cloning, which can not be done with AF's.

Best wishes to you, and all in the community!!!

 

[high life 45]

its a top feed recirculating system, some might call ebb and flow or flood and drain. I never flooded it though.

 

[Loc Dog]

my buddies in straight coco hydroton got it.

That was the item I wanted to eliminate, was coir.

I used to use straight hydroton, and had no problems. Have now boiled everything I use, after the possibility of having an unknown, west coast pathogen. I do not want to become modern typhoid Mary.

It is up to all of us to prevent spread of this crap!!!

I think I am safe, but still want to help give closure to this problem/problems!!!! Still believe it is a few problems, or problems that benefit from each other (Like root nematodes needing damage to take over).

Thanks to all, working on this!!!

 

[Sam_Skunkman]

Was it flood and drain?

That was my favorite, which I used for autoflowers. had total control with central reservoir. Maintain PH, and watch whether PPM went up or down, to know if feeding too much or too little. Found half of GH flora series recommendation was right for 90 day auto flowers.

Did not realize the benefit of cloning, which can not be done with AF's.

Best wishes to you, and all in the community!!!

You can clone AF's first you in-vitro culture them as callus and then root and shoot them and you have the same plant. I know that directly cloning them is hard to impossible, but with a will there is a way. As callus they just ignore photoperiod.
-SamS

 

[Loc Dog]

From Waynes world "I am not worthy"!!!!!!

I appreciate you, and others, with PHD's or Masters in Biology/Molecular Biology/Etc..

Thank You , for all that you do!!!!!!!!!!!!!

You can clone AF's first you in-vitro culture them as callus and then root and shoot them and you have the same plant. I know that directly cloning them is hard to impossible, but with a will there is a way. As callus they just ignore photoperiod.
-SamS

 

[Loc Dog]

its a top feed recirculating system, some might call ebb and flow or flood and drain. I never flooded it though.

Those materials seem inert. I use hydroton, which I now boil between uses.

Thanks!!

P.S.

Do not want to get off topic, but thanks to all who have contributed to the hopeful eradication of this problem!!

 

[paladin420]

Damn good work all!!!
Bunch of Stoners!!!

As I just lurk and certainly ain't sharp enough to understand most of what is going on here I have a question.
Have rapid/riot rooters been looked at as the vector?
Thanks again for all the work

 

[Mikenite69]

Speaking of coco I don't know if roots organic put soil mites in there blocked coco. My last block I just expanded had these little stupid looking white bugs that everytime I watered would float to the top of the coco and if you looked hard enough you would see them move. They had these little antennas that constantly moved with bulbous body's.

Anyone ever see these things before?? Are they beneficial? They would literally be all over the top of the coco. I did a drench of Azamax and they smiled at it. Then I hit them with insecticidal soap and mosquito dunks and those bastards live no more whatever they were.

 

[FtWendy]

just a guess, but those little white guys sound like springtails mike... they're harmless

 

[papaduc]

Has everybody accepted that it is nematodes causing all the issues in here?

If so, on what basis?