Brassinosteroid functions in a broad range of disease resistance

[aus2canibasiva]

Thanks Shaggy for the words of encouragement even if I have to take a step back i'll still be taking steps forward if you know what i mean . & Omera1 does seem to be a knowledgeable fellow from reading his posts in other threads.
Hey unfortunately I live in a country were it is illegal to grow so I cant do side by side experiments due to security problems arising from the potential smell of more plants & the legality of my grow, maybe one day it will be reality, in the mean time I would have to move to America or Canada to get serious, like that's going to happen soon , so unfortunately it is all just "anecdotal evidence!!!" atm, But id'e thought Id'e put it up so people can pick at it and critique it or put them on to a new thought pattern potentially.

Here is the link to my Chitosan, it costs $6 + what ever shipping you'll have to pay:
http://www.ebay.com/itm/Chitosan-Ol...-1-GRAM-for-hydroponics-or-soil-/151182334647

Pictures of Chitosan:



On another note Methyl Jasmonate (MeJa) has been a headache to source in small test size amounts; 1g, I don't want to say impossible.....I am waiting on a msg from one supplier, the same place I got the Atonik. iv'e checked what Google has thrown my way & those sources don't E-mail me back, oh well there loss.

ok, that part is interesting... but 300ppm bras!?

I know Omera1 it is very interesting. Well I did use 100 PPM without detrimental effect so far....., once again it's a waiting game.

Most of my note pad document is copy pasta that I thought merit further attention, I understand that most of it is a mix of right and wrong facts but hopefully some one can come along and make sense of it all, it wont be just one who makes sense of it, it will be thousands of people piecing it together over time, which I like the thought of. Enough rambling.

Plants are still happy today, still praying to the light, when I see the "V" I'm happy to!

Peace.

 

[aus2canibasiva]

Here is a pic of my plants doing the "V"



Here are pictures of various PGS's & Plant Hormones that I have just to show that i'm actually using these compounds, I see to many posts & threads that talk about using plant hormones but don't show any pictures or proof which is unfortunate.

PICTURES

 

[aus2canibasiva]

Sorry about all the pictures being upside down the upload that way it seems, there straight up before i upload.

the Triacontanol I use: from a company called Nutri-Tech Solutions there an Australian company & they have alot of informative papers on everything pertaining to biology plant physiology heaps of diverse stuff,
here is a link:
http://www.nutri-tech.com.au/blog/page/4/



HALO Alpha Beta Protein:



Atonik up close:

 

[aus2canibasiva]

no problem
i dont see a problem to use it in flower, but you have to be careful because of the stretch. like shaggy said you better use a brass/bap combo. 0.01/100/50 brass/bap/thiamine could be a good mix to start imo. good luck, and please report results

Hey Omera1 Iv'e heard that this is the course of action potentially to cut out the stretch completely.

"How To Use 6-Benzylaminopurine:

Example Uses:
150 to 300 PPM: increase in flower size.
300 to 400 PPM: stem elongation reduction.
1000 to 3000 PPM: will cause plant to branch out."

My plants are still stretching but nothing that warrants a freak out maybe the 100 ppm 6-BAP I sprayed is slowing the stretching effect of the 100 ppm Brasinolide, personally i think they've synergised causing a moderate stretch and increased bud set.
But I was also wondering if the 100 ppm of Brassinolide is staying in the plants system? or has it been used up in a relatively quick manner? & if the Brassinolide has stayed in the plants system could I spray any more Brassinolide? because it could have a disastrous effect potentially? or maybe i'm being over paranoid, I do want to use it at week 4 of flower with the 6-BAP to see if it does indeed cause " a dramatic increase in bud growth. Combined with the earlier spraying of Brassinolide in veg, the end result is outstanding in terms of quality and yield."

Peace.

 

[shaggyballs]

Lets start here:
you are using .15% pure BRASS!
how much Weight did you add, and what volume of water did you add it to?

 

[aus2canibasiva]

Alright sounds good,
I added 0.100g to 1 liter of distilled water. Leaving me with 100 ppm.

 

[shaggyballs]

Alright sounds good,
I added 0.100g to 1 liter of distilled water. Leaving me with 100 ppm.

I stumbled through this myself!!!!!!!!!!!
So I feel your pain.
But this can even be conquered by someone with just a few brain cells left!!
I am living proof!!!!!!!!!!!!!!!!
.......
(Laughing at self.)

1g of pure substance per liter = 1000 ppm
0.1=100 ppm this is true

But if your substance is not pure, than you have to account for it's percentage in your case it is 0.15% pure.

So for you 1g in a liter = 1.5ppm
But you added 0.1 grams per liter ...right?
That would be 0.15 PPM.....still 15X stronger than I ever used!!!!
How to mix BRASS.
That is something to be noted!!!!!.
Exactly what day in flower was BRASS applied?
Lets keep this on topic for Omera!!!
I bumped my hormone thread if you would like to read it and discuss other hormones there!!!
I am very eager to see your results even without side by side!!!
Shag

 

[aus2canibasiva]

SHAGGY
Thanks heaps for the calculations your a champ!

Alright here we go:

APPLY BRASS: 14th of Feb. 1 week before flower starts.
START FLOWER: 21st of Feb. start of flower.
APPLIED 6-BAP: 28th of Feb

On the 7th of March Atonik will be applied again with Potassium silicate and fulvic acid.

On the 14th of March I will use more 6-BAP possibly 150 ppm to 500 ppm depending on whether the stretch continues to uncontrollable heights used with Ascophyllum Nodosum used at 1g per liter.
Also I will begin using Chitosan Oligosaccharide at 100 PPM
& another use of Harpin foliar spray. Unfortunately I don't have any Thiamine Omera1.

That's all for now, I have to see how the plants respond and go from there, it's like reading a weather forecast using hormones & PGS's for the first time. It's a great journey of discovery & we as this generation owe it to the future to forge ahead, discover new ideas & new found understanding. that's me anyway, the optimism of youth you could put it down to

OMERA1

About disease resistance, iv'e heard that someone completely cured there powdery mildew problem, by cutting fresh Cannabis leaves soaking them in water for a week or so then they sprayed the cannabis leaf solution on the affected plants & bam it was fixed, I thought that was worth looking into since it seems so simple & think of the ramifications if it did work we could help so many with powdery mildew problems, i'm being a bit optimistic but still seems worth looking into, the only problem is that iv'e never had that problem neither (TMV) Tobacco Mosaic Virus. regardless i'm happy you had good results in fixing your (TMV) congrats!

 

[aus2canibasiva]

If you only have 2 brain cells left there the 2 smartest in the world because i'm having trouble thinking 2 brain cells could do much more than twitch an eye.

Even with all the science it's still the spark of imagination that truly counts and then the initiative. Both of which you seem to have.
Ah in the end you must be just a humble person.

Peace.

 

[shaggyballs]

When people truly work together a lot can be accomplished in a much shorter time!!
@ aus2canibasiva
Thanks for the play by play it will go into my notes and I will try some of it myself and post results!!

"Any who wish to profit himself alone from the knowledge given him, rather than serve others through the knowledge he has gained from learning, is betraying knowledge and rendering it worthless"

 

[aus2canibasiva]

"Any who wish to profit himself alone from the knowledge given him, rather than serve others through the knowledge he has gained from learning, is betraying knowledge and rendering it worthless"

Definitely true, I believe in progression and freedom of information, definitely a good thing.


Yeah will throw notes each others way, check out my photo albums and you can see my Seedism Kill-Watt is stretching from the Brass applications, it is known to stretch even though it is a predominantly Indica strain but the Heavy Afghanica Scott's OG is still remaining squat it seems it doesn't respond or it is at its genetic limit of stretch given the amount of time vegging which was 3 months & 1 week. just a continual observation.

but yeah my albums show everything.

Peace.

 

[aus2canibasiva]

Sorry about the pics being upside down you could always save em to your comp and turn them around in the picture viewer, a bit annoying I know.

 

[shaggyballs]

Sorry about the pics being upside down you could always save em to your comp and turn them around in the picture viewer, a bit annoying I know.

When is aussie are they right side up???

ROFLMAO

 

[aus2canibasiva]

LMAO!

Haha, nah there just like that when they get uploaded, ICMAG should have an edit option so you can crop & rotate you pictures.

So do you reckon I should I use the Brassinolide at the same amount I used it at the first time when I do use it in week 4 of flower?
Do you think the excess amount would have stayed in the system of the plant or would have the plant used it all during it's 14 to 21 day hormone cycle?

I wonder if a plant can reserve excess Brassinolide mmm.....a wild guess, potentially the hypothesis of "reserve excess Brassinolide" is how it staves of infection from a virus or pathogen for it's entire life or either it uses the Brassinolide in a hormone cycle & that facilitates certain processes to set it up to be able to stave of infections viruses etc.

A wild stab in the dark.

 

[Pomodoro]

my growpartner once foliared (unknown concentration) of GA3.

the buds never stopped stretching. be warned.

 

[shaggyballs]

Man you are really on to something now.
Hormones do wear off after a while ...How long you ask?...This is strain dependent for sure I have seen it in my own studies!
Brass works with GIBB that is already in the plant, Gibb makes big fluffy yields also....Brass is very interesting.

If your buds start looking fluffy (they do a little) cut back some.
It is always a trade off Quality vs Quantity a plant can only focus its energy on one or the other when you push it genetically.
You may find the perfect strain that will do both.....but that is a tough one!

As far as ratio goes:

I still recommend >0.01 ppm brass-100 bap 1 week before flower.
Switch lights
Wait 3-7 days if you see too much stretch then add up 100 ppm
BAP alone in a spray.
When stretch is done spray with >0.01 ppm brass-100 bap again.

Again this is strain dependent!!!!!!


I have not sprayed late in flower.
BAP has a waiting period I heard but I have also heard the contrary version.
We smoke it so we don't know for sure!

I would use Brass in feed water.....maybe BAP too...High amounts BAP in feed water can inhibit root growth.
shag

 

[aus2canibasiva]

Hey Pomodoro, cool name.

Back on Post 14 of this thread I quoted this.

I haven't gotten around to using the Chitosan Oligosaccharide, GA-3 witch i don't think i'll use since every report that iv'e read seems to indicate that it needs to be used in such micro amounts that I don't have to tools to dose out something that small like a micro pipette.

I wont be using it. Every report iv'e read has resulted in people wishing they hadn't used it, i'm glad I can learn from others mistakes, since it was on my schedule to use, phew!

Cheers for the conformation of Gibberellin's effects, appreciated.

You maybe able to stop all upward growth by using a large amount of Cytokinin, one called (6-BAP) when used in amounts of 1000 PPM it should retard the upward growth signaling more side branches, it all depends on how much (GA3) was used, which is unknown.

Peace.

 

[aus2canibasiva]

For the post above where I say "(6-BAP) when used in amounts of 1000 PPM" I mean 500 PPM.


Hey Shaggy. that's good to know "hormones wear off after a while", cheers.

"If your buds start looking fluffy (they do a little) cut back some." Is this comment directed at me? if so what should I cut back on?

Once again it's a waiting game and my eyes will be watching keenly , my Lucky Charms & my Killa-Watt are still stretching 2 inch's a day. In 7 days when my plants are in the excepting stage of there hormone cycle on the 14th I will give them 100 to 500 PPM to stop the stretching or reduce it depending on if they stop stretching within the 7 day waiting period, will see soon enough, excited with anticipation

My plant hormone cycles are in a 14 to 16 day periods fyi.

Also I cant "switch lights" because i'm growing outdoor or did you mean natural light switch to do with light decreasing in the Autumn months?
Also everything usually takes about two weeks longer outdoor no instant transition to 12/12.

 

[shaggyballs]

For the post above where I say "(6-BAP) when used in amounts of 1000 PPM" I mean 500 PPM.


Hey Shaggy. that's good to know "hormones wear off after a while", cheers.
Gibb did not seem to wear off all plants died veg/dirt!

"If your buds start looking fluffy (they do a little) cut back some." Is this comment directed at me? if so what should I cut back on?
yes....hard to be sure unless i was there
too late now but next time for the stretchy strain use less BRASS or maybe more BAP !!
You are moving at light speed compared to me .....are you an educated person? Male?(don't want to offend in the future)

Once again it's a waiting game and my eyes will be watching keenly , my Lucky Charms & my Killa-Watt are still stretching 2 inch's a day. In 7 days when my plants are in the excepting stage of there hormone cycle on the 14th I will give them 100 to 500 PPM to stop the stretching or reduce it depending on if they stop stretching within the 7 day waiting period, will see soon enough, excited with anticipation

I too am exited you are using high amounts with good results so far but please be careful!!!

My plant hormone cycles are in a 14 to 16 day periods fyi.

Also I cant "switch lights" because i'm growing outdoor or did you mean natural light switch to do with light decreasing in the Autumn months?
Yes transition
Also everything usually takes about two weeks longer outdoor no instant transition to 12/12.
You may need to play with the timing a bit then.

shag

 

[aus2canibasiva]

Nice Photos Pomodoro!

Nice Photos Pomodoro!


Hey Pomodoro I was looking through your albums & your Dinafem Sweet Deep Grapefruit DAY 50 photo looks amazing! such well formed trichomes!

Did you use any plant hormones in that grow? or any of your grows, any info on that topic would be much appreciated