Inducing Shoots from Callus
- Thread starter mrrangz
- Start date
I have grown 1 shoot in a aero-cloner.
Bap will do it.
Bap will do it.
I was just trying to root in a aero cloner.
All my clones received a accidental dose of 100 ppm BAP right when callus was well formed but yet the cells were still undifferentiated.
The clones were not rooting and I noticed a small shoot (bud site) growing from a cut off branch node.
I did not pursue this course of action as I was trying to get the undifferentiated callus to produce root cells not shoot cells!!!!!
I dosed them with some auxin and removed the shoot growth and 1 week later they had all rooted.
What you are attempting to do to the cutting is to produce undifferentiated callus tissue from meristem cell. The rest is up to the plant. IBA and NAA are not rooting hormones but rather hormones for producing callus from which roots will be created out of the plant's needs.
Use Iba to grow callus then when callus is formed hit them with BAP to tell the undifferentiated callus to produce shoot cells!!!
mrrangz
Member
Thanks for the info shaggy.
Do you have any more information (journals) explaining this action as quoted above?
also rather than using 6-bap, whats your view of shooting it with kt-30 or kinetin?
I have read kinetin is better for younger plants...not sure first hand.
Kinetin is often used in plant tissue culture for inducing formation of callus (in conjunction with auxin) and to regenerate shoot tissues from callus (with lower auxin concentration).
The best Idea I have on what PPM to use is based on a Tomato bloom spray" Cytokinin as kinetin based on biological activity 0.00008%
I recommend trying to coax a shoot out of an area that a branch once grew as that is where mine was produced.
I have read that area contains special cells (what kind)????? I don't know??????
All I know about hormones is here.
https://www.icmag.com/ic/showthread.php?t=263131
Kinetin is often used in plant tissue culture for inducing formation of callus (in conjunction with auxin) and to regenerate shoot tissues from callus (with lower auxin concentration).
The best Idea I have on what PPM to use is based on a Tomato bloom spray" Cytokinin as kinetin based on biological activity 0.00008%
I recommend trying to coax a shoot out of an area that a branch once grew as that is where mine was produced.
I have read that area contains special cells (what kind)????? I don't know??????
All I know about hormones is here.
https://www.icmag.com/ic/showthread.php?t=263131
Maybe I am not using the term callus correctly????
I am speaking of the little bumps that appear on the stem of a plant that soon turn into roots!!!
For me they never appear on the cambium layer....maybe the very bottom or the cutting!!!!
For me they appear without a wound!!!!
In my case that little bump made a small shoot due to the high rate of cytokinin!
Someone take me to school please.
shag
edit
looked around seems everyone else calls it callus.
I know it is callus in tissue culture!
I am speaking of the little bumps that appear on the stem of a plant that soon turn into roots!!!
For me they never appear on the cambium layer....maybe the very bottom or the cutting!!!!
For me they appear without a wound!!!!
In my case that little bump made a small shoot due to the high rate of cytokinin!
Someone take me to school please.
shag
edit
looked around seems everyone else calls it callus.
I know it is callus in tissue culture!
