tissue culture propagation text?

[sunset limited]

So I've been studying up on those ole miss papers where they got their test tube clones up to 95% viability and then made synthetic seeds from the axillary buds. Thoroughly fascinating stuff.

While I haven't read the journals in awhile, it's not a read that's beyond my comprehension. That said, I don't have the background I wish I did with respect to tissue culture in general. There's a dozen (expensive) books available on the subject. I don't know any of them from a can of paint on a shelf though.

Can anyone out there in IC Land recommend a good book that treats tissue culture propagation? And does anyone know whatever became of chimera's foray into synthetic seeds?

THANKS!

 

[N. Accumbens]

Not sure if its what your looking for but theres a chapter on TC, and hopefully (@ 2009) its not outdated.

Biotech of Cannabis

Tc,
Nacc

 

[sunset limited]

interesting read. and although it was published just after this paper:
http://home.olemiss.edu/~suman/Directshootorganogenesis.pdf, it makes no mention of it or the researcher's next paper, dealing with synthetic seeds.

anyway, what i'm looking for really is a book that deals with the practice of tissue culture prop in general and not so much the technique specific to C. sativa. thanks though.

 

[LittleAmsterdam]

What do mean by "Synthetic Seeds"?

Tissue Culture is an advanced technique of cloning. It was originally developed for the Tobacco Industry many moons ago and have been applied to many popular flora that needs to be mass produced or can not be reproduced via typical cloning techniques. i.e. Orchids

 

[LittleAmsterdam]

Imagine a genetic storage bank of 100 test tubes of your favorite plant(s).

Pick your favorite one and grow them out for flower or future seeds.

It is a process and it's not easy. It takes time, patience, and practice to get them successfully into culture and vice versa.

 

[LittleAmsterdam]

A good book that covers the basics is "Plants from Test Tubes: An Introduction to Micropropagation" It's available used from Amazon.

 

[OgRastaman]

I also just started into the vast depths which is tissue culture and knowledge of t.c. maybe these books might be of some help to you sunset I got these as torrents off the internet but they are also text books you could get on paper/hard cover

1) Plant Biotechnology and Genetics
Principles, techniques, and Applications edited by C. Neal Stewart Jr.
2) Dictionary of Plant Breeding 2nd edition (For all plants) By : Rolf H.J. Schlegel
3) Principles of Plant Genetics and Breeding
(For all plants) by : George Acquaah
4) Plants From Testubes : an introduction to Micropropagation 3rd edition By : Lydiane Kyte and John Kleyn

These are the books I am curently studying look forward to the glue at the cup in S.B. hope that these books might help you in your quest to produce the best!!! Glue for 1st in the LA med cup!!

" I never went ro collage but im a scientist at this shit "

 

[Only Ornamental]

My insider tip, especially for those with a university account (or for the print versions a uni library close by): Current Protocols and Nature Protocols
These protocols are great: nearly idiot proof, easy to follow and most of all really work!
You'd have to search for your topic as there are many fields covered and I didn't look .
With a bit of luck, some are free and sometimes they have promotion days where you can access all the older (>5 y or so) protocols .
I had the chance to get nearly all I searched for due to a glitch they had exactly when I was looking for them *g*.

 

[OgRastaman]

Thanks sunset for bringing the cuts to the med cup cant wait til these lil 8 babies grow up.

 

[Banefoul]

I recently read about synthetic seeds. Would be nice to get clone only seed clones. There is a post some years old on here.

 

[xxxstr8edgexxx]

do any of you all know of any supply companies selling preloaded prepared petris for shoot induction/elongation and rooting in standard mediums for t.c.? great links. can you post one for the subsequent synthetic seed paper.

 

[sunset limited]

you asked for it.

http://link.springer.com/article/10.1007%2Fs12298-009-0008-8#page-1

 

[xxxstr8edgexxx]

THanks!

 

[Halonium]

the recipes and hormone concentrations are species specific, so you would have to find a company that makes the petri dishes/flasks on order..

 

[Banefoul]

if you have not had a chance here is the old post.

https://www.icmag.com/ic/showthread.php?t=90236

 

[Infinitesimal]

thanks for starting the thread SL, I need to learn how to use micropropagation as quick as possible!