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Calcium could help .
I had probs with lock out a cpl of Winters ago . The hydro shop guy put me onto some cal mag for foliar . Made a diff over night . My plants were several weeks old and not seedlings so not sure how a seedling will react .
John innes no.1 is terrible shit. I'd use something else. They won't be cold at 22c unless they're going colder in the night.
What's a 5g space bucket?
I don't know if it is actually possible and I said I didn't think it could be phosphorus lockout because seedlings live off their cotyledons but in the interest of knowledge I have to point out that on page 7 of the infirmary link by stitch there is a picture of a seedling with phosphorus lockout due to cold temps.
the first set of leaves it grew one of them had 1/3 missing and now the other set of leaves it's grown one is smaller than the other.here's the link for a space bucket http://www.spacebuckets.com/
why would it be a def in soil at such an early stage, the soil should have alot of nutrients stored in it already. at such an early stage it does not need any nutes.. the soil may have to much nutes in it. is nute burn an option anyone ?