98% THC Budder fact or fiction?

[Gray Wolf]

Whoever gets 95% much less 98% without quality chromatography should verify with a more expensive lab that uses competently-operated GC/MS or HPLC.

Can dispensaries sell distilled and/or chromatographically purified extracts, and, is there a market for them? Someone must be making and selling these, unless you can't, in which case they'd be sold as something else. Either way, the real deal comes with real proof - full analysis documentation from a serious lab.

And consider the implications of everything else that had to be removed to reach that concentration.

 

[shamansgifts]

It trips me out that assholes like shmalphy and Sam sleezy fag are so fucking disrespectful to Grey Wolf, who takes slot if time and care out if what he already does to answer allot of questions people ask 30 times a day. You don't hear him being an asshole , he would instead answer until you understood what he meant. He thought you guys were some dip shits that have remarkably similar personalities of other well known trolls/dumbfucks. And normally im the quiet one!. You guys both just look like idiots even talking to him like that . Anyways the isomerization to acetate worked well in commercial.cannabis,Schwag and hemp like types found years ago. It turned the mostly cbd containing plants into thc. With todays high thc strands to start there isn't much to convert. However acetate will.break the blood brain barrier much faster than normal making it seem slightly more potent. If I could id put money on an absolute decarboxylated sample would probably have the most impressive test results. More than 90%purity would probably be quite a taste drop as pure actives are mostly Colorless and odorless. We shall soon see, I hear someone has there own Gc.!

 

[shamansgifts]

I know I got in really late so sorry for the late rant. I'm in Colorado and lots of places claim that they test so I'll see if I can post some. But im on a mobile and it won't let me upload sadly I don't know computers. C

 

[Waxmasta Flex]

It trips me out that assholes like shmalphy and Sam sleezy fag are so fucking disrespectful to Grey Wolf, who takes slot if time and care out if what he already does to answer allot of questions people ask 30 times a day. You don't hear him being an asshole , he would instead answer until you understood what he meant. He thought you guys were some dip shits that have remarkably similar personalities of other well known trolls/dumbfucks. And normally im the quiet one!. You guys both just look like idiots even talking to him like that . Anyways the isomerization to acetate worked well in commercial.cannabis,Schwag and hemp like types found years ago. It turned the mostly cbd containing plants into thc. With todays high thc strands to start there isn't much to convert. However acetate will.break the blood brain barrier much faster than normal making it seem slightly more potent. If I could id put money on an absolute decarboxylated sample would probably have the most impressive test results. More than 90%purity would probably be quite a taste drop as pure actives are mostly Colorless and odorless. We shall soon see, I hear someone has there own Gc.!

yes gdub is the man...ive been telling people to answer posts like him for the last year!! lol i was trying to post a pic of my highest test results showing over 95% cannabinoids..but not letting me save it...ill post tho as soon as can...
im sticking with what ive seen...its possible you just gotta use super fire colas and know what ur doin and u can get some pretty damn strong shit....

 

[devilgoob]

graywolf, you think centrifuges can provide the thrust to let flow cannabinoids past other cannabinoids so that they are separated by weight?

also drying or curing is important for a pure result. I believe all the volatilles and smaller cannaboids are gone after six months of drying in a semi-enclosed space, leaving a person with a higher concentration of THC per weight. It would however take away from the personality of the high and from the effects, the entourage effect.

 

[Gray Wolf]

graywolf, you think centrifuges can provide the thrust to let flow cannabinoids past other cannabinoids so that they are separated by weight?

also drying or curing is important for a pure result. I believe all the volatilles and smaller cannaboids are gone after six months of drying in a semi-enclosed space, leaving a person with a higher concentration of THC per weight. It would however take away from the personality of the high and from the effects, the entourage effect.

I don't see a centrifuge able to do that.

We dry for about 5 days for the best flavor retention and entourage effects. Longer drying will produce prettier extractions, but they will lose many of the mono and sesquiterpenes.

 

[madchemist]

Please see Chapter 3 of Arno's thesis regarding centrifugal separation of cannabinoids. This probably isn't exactly what you were thinking devilgoob, but it's the best answer I have for now.

http://scholar.google.ca/scholar_ur...r&ei=cvAJUYScJ4mJiwKb5oGwAQ&ved=0CC4QgAMoADAA

"Preparative isolation of cannabinoids from Cannabis sativa by centrifugal partition chromatography

••• Arno Hazekamp, Ruud Simons, Anja Peltenburg-Looman, Melvin Sengers, Rianne van Zweden, Robert Verpoorte •• Leiden University, Department of Pharmacognosy, Gorlaeus Laboratories Leiden, The Netherlands • Published in J. Liq. Chrom. Rel. Technol. 2004, 27(15): 2421-2439

Abstract
A simple method is presented for the preparative isolation of seven major cannabinoids from Cannabis sativa plant material. Separation was performed by centrifugal partition chromatography, a technique that permits large scale preparative isolations. Using only two solvent systems, it was possible to obtain purified samples of the cannabinoids; (-)-∆9-(trans)- tetrahydrocannabinol (∆9-THC), cannabidiol (CBD), cannabinol (CBN), cannabigerol (CBG), (-)-∆9-(trans)-tetrahydrocannabinolic acid-A (THCA), cannabigerolic acid (CBGA) and cannabidiolic acid (CBDA). A drug-type and a fiber-type cannabis cultivar were used for the isolation. All isolates were shown to be 90-95% pure by gas chromatography. This method makes new cannabinoids available on a large scale for biological testing. The method described in this report can also be used to isolate additional cannabinoids from cannabis plant material.

...

3.4 Conclusion
Preparative isolation of seven different major cannabinoids could be achieved by using CPC as the single technique, with two different solvent systems. The quality of the isolated cannabinoids (>90% pure by GC-FID) is sufficient for many purposes. Additional HPLC and TLC data support the purity of the isolated compounds. This method can make the isolated cannabinoids available for biological testing on a large scale. Also other cannabinoids can probably be isolated in this way by choosing a cannabis variety with a high content of the desired cannabinoid and simultaneously a low content of cannabinoids that are known to overlap with the desired cannabinoid in the CPC separation. The vast diversity in cannabis varieties should make it possible to find a suitable variety for most cannabinoid isolations. It should be possible to isolate several cannabinoids in just one chromatographic run, but the efficiency depends on peak overlap and contamination of the sample with non-cannabinoids. To ensure a high yield the acidic cannabinoid fraction of a cannabis extract should be used."



I've talked with scientists who told me how cool centrifugal chromatography is and I've seen the instrumentation used. That being said, I've never seen it used with cannabinoids or heard of anyone in North America who has. Arno claims it's scalable to prep scale...

 

[dybert]

Please see Chapter 3 of Arno's thesis regarding centrifugal separation of cannabinoids. This probably isn't exactly what you were thinking devilgoob, but it's the best answer I have for now.

http://scholar.google.ca/scholar_ur...r&ei=cvAJUYScJ4mJiwKb5oGwAQ&ved=0CC4QgAMoADAA

"Preparative isolation of cannabinoids from Cannabis sativa by centrifugal partition chromatography

••• Arno Hazekamp, Ruud Simons, Anja Peltenburg-Looman, Melvin Sengers, Rianne van Zweden, Robert Verpoorte •• Leiden University, Department of Pharmacognosy, Gorlaeus Laboratories Leiden, The Netherlands • Published in J. Liq. Chrom. Rel. Technol. 2004, 27(15): 2421-2439

Abstract
A simple method is presented for the preparative isolation of seven major cannabinoids from Cannabis sativa plant material. Separation was performed by centrifugal partition chromatography, a technique that permits large scale preparative isolations. Using only two solvent systems, it was possible to obtain purified samples of the cannabinoids; (-)-∆9-(trans)- tetrahydrocannabinol (∆9-THC), cannabidiol (CBD), cannabinol (CBN), cannabigerol (CBG), (-)-∆9-(trans)-tetrahydrocannabinolic acid-A (THCA), cannabigerolic acid (CBGA) and cannabidiolic acid (CBDA). A drug-type and a fiber-type cannabis cultivar were used for the isolation. All isolates were shown to be 90-95% pure by gas chromatography. This method makes new cannabinoids available on a large scale for biological testing. The method described in this report can also be used to isolate additional cannabinoids from cannabis plant material.

...

3.4 Conclusion
Preparative isolation of seven different major cannabinoids could be achieved by using CPC as the single technique, with two different solvent systems. The quality of the isolated cannabinoids (>90% pure by GC-FID) is sufficient for many purposes. Additional HPLC and TLC data support the purity of the isolated compounds. This method can make the isolated cannabinoids available for biological testing on a large scale. Also other cannabinoids can probably be isolated in this way by choosing a cannabis variety with a high content of the desired cannabinoid and simultaneously a low content of cannabinoids that are known to overlap with the desired cannabinoid in the CPC separation. The vast diversity in cannabis varieties should make it possible to find a suitable variety for most cannabinoid isolations. It should be possible to isolate several cannabinoids in just one chromatographic run, but the efficiency depends on peak overlap and contamination of the sample with non-cannabinoids. To ensure a high yield the acidic cannabinoid fraction of a cannabis extract should be used."



I've talked with scientists who told me how cool centrifugal chromatography is and I've seen the instrumentation used. That being said, I've never seen it used with cannabinoids or heard of anyone in North America who has. Arno claims it's scalable to prep scale...

Arno validated my companies' test protocols

 

[Ilikecookies]

I wonder what the genetics to that watty kush were. I know the mother has been lost which totally sucks but I heard that the bubba kush and purple kush coming from from that area, maybe from TUG, is supposed to be just as good for concentrates. Maybe it is just TUG's growing style?

I just wish I could try and compare the BK budder to the Cali wax.

The BK budder (made from buds) came in 3rd place at the Kush Cup in Vancouver this last year, beaten by budder that the guys said was made from trim (not buds).

The Watty Kush was supposed to be from Afghanistan in the 80's, genetics unknown. It's still around.

 

[Gray Wolf]

"Preparative isolation of cannabinoids from Cannabis sativa by centrifugal partition chromatography

http://en.wikipedia.org/wiki/Countercurrent_chromatography

 

[Wendull C.]

I got some 95% wax just to try it next to my girl scout cookie shatter. I was skeptical but one dab knocked me on my ass, whether the test was inflated idk but it was very potent and the test was from sc labs.