What's new
  • Happy Birthday ICMag! Been 20 years since Gypsy Nirvana created the forum! We are celebrating with a 4/20 Giveaway and by launching a new Patreon tier called "420club". You can read more here.
  • Important notice: ICMag's T.O.U. has been updated. Please review it here. For your convenience, it is also available in the main forum menu, under 'Quick Links"!

How do you process moldy buds?

purple_man

Well-known member
Veteran
id say: discard them...
many moons ago i recieved some very moldy/semi wet pound from a bro who wanted to know if we could make bubble out of it...

we tried it, and threw away everything...
safety/health comes first in my opinion!!!

blessss
 

LiLWaynE

I Feel Good
ICMag Donor
Veteran
Also, mold spores range in size from 1 u to 30 u. This means that they will flow through any size bag with a number larger than that. This means you can process moldy bud into bubble hash using the 45 u bag as the smallest bag and the mold spores will flow through that bag into your waste water. I actually used the 25 u bag as my smallest bag for this latest run and just gave everything a very good rinse.

Also, the actual mold filaments, the fibrous stuff you see on your buds will either get trapped in the larger bags (220 or 190), or if you wait long enough they will float to the top of the water while the trics sink to the bottom



OK.....

The most common mold spores found within a moldy bud WILL FLOAT.....


trichs fall to the bottom and go through the screens....that is how it works... the mold spores are too light to fall through the various sized silk screen along with the trichomes...
 
B

BrnCow

My buds had that happen once. He very carefully picked out the molded stuff (young eyes- I couldn't do it anymore) and threw that away. Then he spread the rest out on newspapers and took vodka, put it in a fine spray bottle and sprayed the rest until it was damp. Then he took it and set it about a foot under the discharge of the window ac on high speed. After about an hour of blowing cold air over it, the vodka was gone. Then he smoked it. But maybe a person could extract it at that point. The vodka might kill the mold. Maybe use the dry ice to extract it, pour it in ice cold water, and pour off the stuff that floats. Hell, try some things and look at the results under a lighted microscope. Don't smoke on it till you get a clean result, if you do. Look at some not molded stuff to compare it to. In this world of chemistry, there is very likely something that will separate the mold from the hash. Freeze it?Let us know when you find the answer...don't forget to take photos at every step so we can see some proof... hell, you might even blow the spores off with a can of computer air...lol .. and don't give it to anyone that is not made aware of the mold. Hope it doesn't make you or your buds sick...
 
Last edited:

HempHut

Active member
I work in a large research lab, and when I get a chance (not until next week) I will try and culture some of the hash I made from moldy buds and see what I can get to grow.


This is a great idea and should provide a bit of data on what's happening in the finished product -- what might actually still be in there that's viable, if anything. I look forward to seeing what comes of this attempt.
 

Gray Wolf

A Posse ad Esse. From Possibility to realization.
Mentor
ICMag Donor
Veteran
Good idea looking at controls and variables! I look forward to the Petri dish results as well and hope you will post some nice macros.

One variable is of course that mold spores of any given variety is or is not present.

If the first moldy sample is Aspergillum, it will prove the point that it can be present, but if it is something else, it unfortunately doesn't prove that it can't be present.

What culturing the moldy hash will show is what strain was present in that specific sample.

Culturing a non moldy hash sample will show how common any mold spores are in un-moldy hash if it blossoms. If it is the same strain, it will add more weight to which strain is most common in the location the samples are taken from and run.

To be statistically significant in demonstrating that Aspergillum is not found in hash, of course many more samples world wide are required on this internationally significant mix of different locations.

The samples would also have to be kept sterile until placed in a culture under a laminar flow hood or equivalent, because exposing them to air invites other mold spores. For instance we have grown both common bread mold and penicillin in tissue culture samples that were not kept adequately sterile.

In point of fact, the moldy hash may have picked up the mold due to exposure to air after it was made and just had excessive moisture content, so it sprouted.

Aspergillum is one of the principle molds composting plant material, so if your plants are anywhere near compost that is still composting, they almost certainly have been exposed to their spores.

I though about adding some spice to this discussion by wagering on which mold it would be, but then it dawned on me that given all the possibilities, I wouldn't have a clue and the odds are way worse than flipping a coin just for the sake of gambling.

Anyone else care to wager a pint of beer to be drunk symbolically on what strain the first sample will be?
 

Gray Wolf

A Posse ad Esse. From Possibility to realization.
Mentor
ICMag Donor
Veteran
Anybody have a source for the .2 micron filters mentioned?

I use syringe filters and buy them at the local American Scientific Supply or pick them up off of E-Bay.

You can also pick up a syringe at the same places. I prefer glass syringes, because I can also use them to suck up and dispense hot oil withour losing the piston head if it cools too much.

PS: You won't be able to filter to 0.2 microns without pressure or vacuum of some sort, as it is in the microfiltration range and blanks off almost immediately and stops flowing.
 
Last edited:

Gray Wolf

A Posse ad Esse. From Possibility to realization.
Mentor
ICMag Donor
Veteran

Attachments

  • Syringe filter.jpg
    Syringe filter.jpg
    11.4 KB · Views: 6

mrcreosote

Active member
Veteran
Grey Wolf,
I always enjoy your posts. Informative and always backed with solid science in a world of hearsay and unsubstantiated opinion. You are a boon to the community and I thank you for being generous with your knowledge to keep us lesser mortals from running amok.
Bear with me as my grasp of science extends not much further than the construction of stink-bombs in high school lab class.
After a microscopic examination to determine that aspergillis is not present and the infected bud is affected with no more than common powdery mildew or botrytis, an ethenol wash/soak would kill active mold spores and a .02 micron filtering would remove their dead carcasses from solution, thus rendering the result safe for personal consumption by a healthy individual?
Could one assume then that vaporizing the resulting oil would add an additional layer of safety by killing any remaining spores in the event of cross contamination by carelessness in the process?
In short, just how sturdy are these simple molds to disinfection?

Chucking out lbs. of weed for something that can easily be remedied with care would be like killing my wife because she came down with a cold.
More often than not, I would seek an alternative modus operendi :)

PS. If you have a resource link handy that explains different simple vacuum filtering processes and equipment for dummies outside of a syringe filter, I and, I'm sure many others, would be greatly indebted to you.
Would a funnel, .02 filter paper, sidearm flask and a mitymite type vacuum pump be sufficient to process larger quantities of solvent than a syringe can? I'm assuming that a more dilute solution filters faster than a concentrate. Ideas for small scale or is a syringe fine for a quart mason jar quantity.
I'm asking for filtering clean bud. I'd need to get a lot smarter to risk messing with moldy stuff.
I'm a firm believer in not messing with dangerous stuff unless you are absolutely certain you know what you are doing.
Don't ask how I learned this... :)

Weed...turning stoners into scientists.
 

Tokesy

Member
Would it be safer to make edibles from hash made from moldy buds rather than smoke it, or would the potential risk be about the same?
 

Gray Wolf

A Posse ad Esse. From Possibility to realization.
Mentor
ICMag Donor
Veteran
Grey Wolf,
I always enjoy your posts. Informative and always backed with solid science in a world of hearsay and unsubstantiated opinion. You are a boon to the community and I thank you for being generous with your knowledge to keep us lesser mortals from running amok.
Bear with me as my grasp of science extends not much further than the construction of stink-bombs in high school lab class.
After a microscopic examination to determine that aspergillis is not present and the infected bud is affected with no more than common powdery mildew or botrytis, an ethenol wash/soak would kill active mold spores and a .02 micron filtering would remove their dead carcasses from solution, thus rendering the result safe for personal consumption by a healthy individual?
Could one assume then that vaporizing the resulting oil would add an additional layer of safety by killing any remaining spores in the event of cross contamination by carelessness in the process?
In short, just how sturdy are these simple molds to disinfection?

Chucking out lbs. of weed for something that can easily be remedied with care would be like killing my wife because she came down with a cold.
More often than not, I would seek an alternative modus operendi :)

PS. If you have a resource link handy that explains different simple vacuum filtering processes and equipment for dummies outside of a syringe filter, I and, I'm sure many others, would be greatly indebted to you.

Weed...turning stoners into scientists.

Thanks for the good thoughts bro!

Vaporizing either powdery mildew or botrytis even without filtering it, is not toxic, though it tastes and smells like shit.

Vaporizing is an excellent way to actually taste the nuances of the different oils, and to insure by testing that all the mold is gone. Alas if aflatoxins from aspergillum are present however, it adds no protection.

Solly I don't have a how to vacuum filtration link, but that is a good point! I will put something together and post some ways to do so on the cheeeep.

In brief, besides picking up a vacuum pump, you can use a simple faucet vacuum aspirator, which you can find on E-Bay, and make a vacuum flask with any flask or bottle, and a two hole stopper.

A Buchner funnel (E-Bay), or DYI equivalent, will accommodate filters (E-Bay), and goes in one hole of the stopper, and the line to the vacuum aspirator goes in the other. Simple, cheeep, and efficient...........
 

Gray Wolf

A Posse ad Esse. From Possibility to realization.
Mentor
ICMag Donor
Veteran
Would it be safer to make edibles from hash made from moldy buds rather than smoke it, or would the potential risk be about the same?

The key issue is what kind of mold, because they aren't all created equal. If it is one producing an aflatoxin, medibles are no protection.
 

mrcreosote

Active member
Veteran
Thanks Grey,
Wow, you're omniscient! Your last post here was almost 2 months ago.
See? this is where filtration dumminess comes into play... I thought a Buchner filter was for separating and keeping solids because the filter just sits on top of a grid possibly allowing a small percent to escape to the solution below (as if the solution were waste). So you can fill a Buchner funnel to almost capacity and it will still work?
Being cheep I thought a $30 hand pump would be better than buying a water aspirator and a second side flask for a water stop...Am I getting the idea or am I hopelessly befuddled?
Would a syringe filter be practical for a quart jars worth, say 20 0z. of iso or ethanol?
I just looked at a flask, Buchner funnel and pump setup on ebay for $50.Seemed like a fairly good deal as I'm finding out that lab-ware isn't cheap. I'll go back and add a link if I can find it again.
Voila:
http://www.ebay.com/itm/Filter-Set-...332?pt=LH_DefaultDomain_0&hash=item2c609cbf3c
It doesn't say what the paper that comes with it will pass but I'm sure Whatman has what it takes,
9cm @.02. micron.
In a few weeks I'll have some nice bud & trim to make fresh material amber and being able to filter it properly would be a real plus.
If you feel a silent toast coming your way around then...
It'll be me having a very merry Christmas.
 
The key issue is what kind of mold, because they aren't all created equal. If it is one producing an aflatoxin, medibles are no protection.

in all fairness, most are not toxic and can be cleared with an ethanol dilution and filtration. a lab is the only way to tell for sure though.
 

Gray Wolf

A Posse ad Esse. From Possibility to realization.
Mentor
ICMag Donor
Veteran
Thanks Grey,
Wow, you're omniscient! Your last post here was almost 2 months ago.
See? this is where filtration dumminess comes into play... I thought a Buchner filter was for separating and keeping solids because the filter just sits on top of a grid possibly allowing a small percent to escape to the solution below (as if the solution were waste). So you can fill a Buchner funnel to almost capacity and it will still work?
Being cheep I thought a $30 hand pump would be better than buying a water aspirator and a second side flask for a water stop...Am I getting the idea or am I hopelessly befuddled?
Would a syringe filter be practical for a quart jars worth, say 20 0z. of iso or ethanol?
I just looked at a flask, Buchner funnel and pump setup on ebay for $50.Seemed like a fairly good deal as I'm finding out that lab-ware isn't cheap. I'll go back and add a link if I can find it again.
Voila:
http://www.ebay.com/itm/Filter-Set-...332?pt=LH_DefaultDomain_0&hash=item2c609cbf3c
It doesn't say what the paper that comes with it will pass but I'm sure Whatman has what it takes,
9cm @.02. micron.
In a few weeks I'll have some nice bud & trim to make fresh material amber and being able to filter it properly would be a real plus.
If you feel a silent toast coming your way around then...
It'll be me having a very merry Christmas.

The Buchner just holds the filter paper, for which we use a #1Whatman lab filter. Depending on how many solids you are filtering out, it may clog and require replacement or you may be able to just keep wiping it off while under vacuum to keep it flowing.

Sorry, the link doesn't open up for me, but I would get a vacuum pump that you don't have to hand pump, if you have much material to process. They are cheeep and easier on the carpal tunnel.

It doesn't take long to process a quart of liquid using a syringe filter. I pre-filter it first at a coarser micron level, to save on syringe filters.

A toast back! Hope you have a memorable Christmas this year!
 
Top