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Lactobacilli question

Zendo

Member
Hey guys,

Making my first culture, and have a question or two.

In the guide posted by Jaykush in OFC, it says:
Lactic acid bacteria will gather in 5-7 days when temperature is 20-25 degrees C. Rice bran will be separated and float in the liquid, like a thin film, smelling sour. Strain and simply get the liquid. Put this liquid in a bigger container and pour ten parts milk.
The original liquid has been infected with different type of microbes including lacto bacilli.
And in order to get the pure lacto bacilli, saturation of milk will eliminate the other microorganisms and the pure lacto bacilli will be left. You may use skim or powdered milk, although fresh milk is best. In 5-7 days, carbohydrate, protein and fat will float leaving yellow liquid (serum), which contain the lactic acid bacteria. You can dispose the coagulated carbohydrate, protein and fat, add them to your compost pile or feed them to your animals. The pure lactic acid bacteria serum can be stored in the refrigerator or simply add equal amount of crude sugar (dilute with 1/3 water) or molasses.

question...after straining the rice bran and adding the 10 parts milk, does that then sit in a cool dark place with loose fitting lid again, or does it go in the fridge?

question 2... Is there any risk here in this process with infections? My wife's friend scratched a zit after doing recycling, and got a superbug that almost killed her.. Dr's said it was from a rotten milk container.
I have told said wife that all is fine, but she is insisting that I find out for sure.

Thanks for any help.
 

big ballin 88

Biology over Chemistry
Veteran
I believe the milk goes stays in a nice cool place. I dont know if it has to be dark though i wouldnt imagine so. Lacto bacillus isn't as nasty as other bacteria's out there regardless you should always was your hands when dealing with organics. Any microorganisms that can infect a soil could probably infect you. I make sure to wash my hands after dealing with soil, compost, worms or even mixing amendments. You take good care of your plant, why wouldn't you take precautions for yourself?
 

jaykush

dirty black hands
ICMag Donor
Veteran
have you been to the lacto b thread? there is full instructions and a lot of discussion.
http://icmag.com/ic/showthread.php?t=96325

but to answer your question. if done right, this is safe enough be used as a medicine. i know a few who have gargled the final culture to get rid of a sore throat. these micro organisms are in the air you breathe because that's where the culture comes from, your just concentrating them and multiplying them. the milk selects for lactic acid bacteria. most recycling bins are set and go bad, left to rot for days on end. thats not the goal with LAB ( lactic acid bacteria)
 

Zendo

Member
have you been to the lacto b thread? there is full instructions and a lot of discussion.
http://icmag.com/ic/showthread.php?t=96325

but to answer your question. if done right, this is safe enough be used as a medicine. i know a few who have gargled the final culture to get rid of a sore throat. these micro organisms are in the air you breathe because that's where the culture comes from, your just concentrating them and multiplying them. the milk selects for lactic acid bacteria. most recycling bins are set and go bad, left to rot for days on end. thats not the goal with LAB ( lactic acid bacteria)

Jay, I had not seen that, but have now read from front to back. Good reads, and all questions answered.

I wish I would have read that thread FIRST, as I ended up with a cup of wash, and then added 10 cups milk..Needless to say, I'll have enough...:)

I'll try to use it in as many areas as I can, and dump the rest on my outside gardens.
 

h.h.

Active member
Veteran
I used coconut milk instead of milk, threw in a banana and actually developed a bit of a taste for it. Sounds like her friend contacted staph which has nothing really to do with milk. What you're basically doing is making yogurt.
 

CannaExists

Paint Your DreamStrain
Veteran
I used coconut milk instead of milk, threw in a banana and actually developed a bit of a taste for it. Sounds like her friend contacted staph which has nothing really to do with milk. What you're basically doing is making yogurt.

Would coconut milk "select for lactic acid bacteria" like jaykush says cow's titmilk does? I'm uber interested in that for I choose not to use animal products. That banana concoction sounds delicious, I'd for sure dine on that.

jaykush you got a minute? Yeah me and h.h. were just talking about... oh right you can see our conversation. What say you?
 

h.h.

Active member
Veteran
It worked for me. The science, I don't know. In a different environment, it might be different.
The lacto B lives off of the lactic acid while other things don't. CC milk contains the about the same concentration of lactic as milk.
I have made it this way a few times and have confidence in the method.
The cc milk I find has potassium bisulfate as a preservative which may or may not have affected the outcome. I don't think it did.
As a disclaimer I must say I have no formal knowledge, don't have a microscope,blah, blah, blah. Only my own experience.
Google coconut milk yogurt.
 

mad librettist

Active member
Veteran
Hang on!

Lactobacilli do not live off of lactic acid. They need sugar like most bacteria. Milk is packed with a sugar called lactose that is quite sweet. When it's all eaten, the sweetness is gone. Now does yogurt make sense. Lactic acid is the waste product.

As for milk inhibiting other bacteria, I don't know. But I DO know that the lactobacilli themselves inhibit other microbes. These guys are everywhere. All over your hands and face and everywhere else. If you get rid of them, expect yeast infection and digestive issues.

Any old sugar will feed afaik. I use molasses.
 

h.h.

Active member
Veteran
Good thing I had a disclaimer. All I know for sure is I followed Jay's method using barley and cc milk as substitutes and it worked.
I should have said "lactose" when I said "lactic acid" as well. CC milk contains a high level of lactose.
Thanks for the correction.
I think lacto B lives rather specifically off of lactose, hence the name.
 

mad librettist

Active member
Veteran
I think it got the name due to human history. Probably first isolated from milk. Lactose is uncommon as a human food worldwide beyond childhood, and there is no lactose on your hands. Yet there they are...

Think "milkbacillus". But it's the same genus responsible for sauerkraut, salami, and a host of other great stuff. Dairy is actually an exception.

Coconut milk - are you sure? I don't know for sure, but i don't think of lactose as a plant sugar. And remember, lactose and lactic acid don't go hand in hand. If your body makes the enzyme lactase you can turn it into glucose, not lactic acid, which is a fuel used in anaerobic respiration in the muscle tissue (before you correct me look it up - the science has been updated)


Edit: hang on again! If science was wrong about lactic acid in anaerobic respiration in a workout, am I out of date when I say it's waste in LAB? I need to mention as well that I was told the acid is also the inhibitory agent.
 

mad librettist

Active member
Veteran
Btw I need to use a disclaimer more often. I always assume it is implied, but then again no one can see my face or hear my voice. So how do they know I'm bantering, blowing smoke, giving confirmed fact, being pedantic, etc... Without seeing me?

So people use imagination to fill in the gap. It's not you they see, it's their own hopes and fears.

Good on you bro, that's a good example you set.
 

h.h.

Active member
Veteran
I don't know enough to correct anybody.
Since Lacto B can be captured from the air, grain, ant hills, it makes sense that lactose is not the only food source..
Actually it is lauric acid I was thinking of that is in coconut. I did add molasses to my mix. Perhaps why it worked.
 

mad librettist

Active member
Veteran
Well coconut milk is pretty sweet to me... It's made from young coconut, no? That's Sugar fest. The wife and I bust them open , drink the juice, the I cut it in two an we eat the flesh.

We call it nature's flan.
 

h.h.

Active member
Veteran
I found this.Using young coconut water.
http://bodyecology.com/mcoconutkefir.php
I used canned cc milk, already pasteurized, and ended with the same product. The grain wash introduced the LB culture rather than a store bought product.
A side benefit is the separated fats make a nice cream for the hands.

The milk is different, made from the white mature flesh and hot water.
 

mad librettist

Active member
Veteran
Probably tasty too...

Young coconut juice (not pressed from the flesh) is pretty clear, and very very sweet. Younger is sweeter but very little flesh. As it matures the flesh gets thicker and sweetness goes down. I always buy the heaviest ones as they are sweeter. I'm hungry and thirsty now!

I'd say your instincts told you to add molasses and banana. So you must have good instincts.
 

jaykush

dirty black hands
ICMag Donor
Veteran
cannaexits and hh, im not too sure on the coconut milk. i have never tried it with that before. im assuming if you get the separation reaction that there is some life in there to do so. as to if its LAB, i cant say. coconut milk is too precious here to use on stuff like this, it all goes to curry lol. now days i am using raw milk from a friends cow.
 

h.h.

Active member
Veteran
I've used the young cc water in making EWC tea, breaking up the shells and throwing them into my soil. After pulling some males, I noticed the roots covered meat side of the shell. Seems they like the flan as well.
 

h.h.

Active member
Veteran
Jay,
Do me a favor and try it. I would like an opinion of someone with more expertise to give an opinion. Reading up, it sounds like the lauric acid(hope I finally got that right) actually inhibits the LB though I felt I was getting the same positive benefits from the final product.
 
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