Plant Tissue Culture Kit?

[accessndx]

The storage of genes via callus is done at lower temperatures with low oxygen levels so that the metabolisms of the plants slow down. Ideally, it is done by cryopreservation which is basically a culture which is frozen and kept that way and melted when time comes to reproduce it, but that is still very hard to accomplish I think. Still, easier than freezing humans

I know this thread hasn't gotten much love lately, but I gotta say K+++ for the posters who previously contributed. The questions I have are simple: is there in fact a way to preserve the cultures so that you don't have to propagate immediately? IE-can you put this stuff in cryogenic environments and save indefinitely? What is the upper echelon of time these cultures are viable for? Can they be saved for years?
I noticed there are a plethora of these tissue culture kits available. It's been a few years since the prior comments were made...has this stuff evolved to a level where it's a bit more accessible for the masses?

 

[SoloGro57]

Subscribing and bumping this thread. There must be people doing this. Talk about it!

 

[JWP]

A good way to get "elites" from far away places

 

[medi-useA]

I have been tossing this idea around awhile now...I'm not going to go into the full on explant/callus routine...I was thinking just of putting sterilized cuttings with an internode and growth shoot into sterile jars with an agar gel containing B-Vitamins and rooting gel...see if I can get roots going and how fast/slow it could be done...I'm hoping to be able to get 4 or 5 cuttings per jar, rooted, but in stasis, ready to be bought out and grown up at a moments notice...I have experimented with the agar gel to get the consistancy I'm after...I'm getting close to taking my first batch of cuttings and seeing what will happen...


muA

 

[GivaDogaBone]

Good info on this sites.

http://www.kitchenculturekit.com/tcinfo.htm

http://www.kitchenculturekit.com/StiffAffordablePTCforhobbyists.htm

 

[Ol'Boy]

Hello, i was lurking on the net an found this..http://www.scribd.com/doc/14571756/The-Biotechnology-of-Cannabis-Sativa

Very informative.. seems the possibilities are endless with PTC.
*note* to save to your pc., u must register for an account which is free btw

Anyhoo hope this is helpful to some of you mad scientist's
Be safe.. an enjoy.

Goodday..Ol'Boy

 

[sophismata]

Correct me if I'm wrong here but wouldn't this be an awesome technique for propagating autoflowering varieties? Sure the first batch would be a long wait but then you could have a perfectly cloned set of autos for your next planting.

 

[medi-useA]

I have doubts about using autoflowers for this...but they are as yet unfounded.

muA

 

[felderosa]

http://www.planttc.com/

was featured in high times

edit: sorry missed the previous post with planttc... either way, im getting the kit for next spring, seems like a decent outfit, but might take a while to get a bunch started.

 

[ChaosCatalunya]

http://www.planttc.com/

was featured in high times

edit: sorry missed the previous post with planttc... either way, im getting the kit for next spring, seems like a decent outfit, but might take a while to get a bunch started.

LOL, been looking for the PTC leaflet I got at Spannabis... turns out it was planttc.com.......

The leaflet does say "Now distributed by BWGS, Micro Hydro and Everest" . if that helps anybody.... I guess Stateside as I do not recognise them as EU distributors.

 

[lovenrg420]

has anyone considered or tried using tc with meristem tissue to remove viruses. I know some have posted saying its a cal/mag def, but Im convinced the OG cuttings going around cali's med dispensaries has a mosaic virus and others probably do to, this method might be able to eliminate such viruses, boosting yields and resulting in a hardier plant.

 

[SoloGro57]

thatd be cool

 

[arrg]

has anyone considered or tried using tc with meristem tissue to remove viruses. I know some have posted saying its a cal/mag def, but Im convinced the OG cuttings going around cali's med dispensaries has a mosaic virus and others probably do to, this method might be able to eliminate such viruses, boosting yields and resulting in a hardier plant.

I don't know if that would work very well because the tiny amount at the tip just might take and without a real node I doubt it would take. here is a little linky to some orchid tek.

http://www.orchideenvermehrung.at/cgi-local/framebreaker/reload.pl?english/lfh/index.htm

I have seen some people work in steam above a pot but a nice laminar flow hood works the best. Find a filter on fleabay and garbage pick a blower out of a furnace and you are good to go. If they legalize mota by me someday I will give it a try.

 

[LEDGro]

Here is an article in urban gardener about tissue culture:
http://urbangardenmagazine.com/2008/10/plant-tissue-culture-for-gardeners-as-well-as-geeks/

Tissue cultures seem very similar to cloning and culturing mushrooms. They also need sterile environments. If I was able to do that in a bathroom I don't see how tissue cultures would be that different.

 

[christen23]

Ive looked into it greatly, and what i found out is that every plant species needs different levels and different "recipes" to make them clone correctly, There is a HUGE group on yahoo that talks about tissue culture and they have a list of plant species and the "recipe" to get them to clone, problem is there is nothing for cannabis sativa... This would be VERY useful in keeping many different strains, If anyone finds out the recipe and does this successfully please let me know as I have great ideas in implementing this in the cannabis community, Shit you could keep literally over 1 million strains in a room as apposed to keeping 1,000,000 different strains in "mom" form, anyways thanks
~POE~

 

[pipeline]

tissue culture is not realy used to preserve the gene pool... During regeneration of callus tissue, there can be some mutations that occur and some of the resulting plants can have altered phenotype...

 

[pipeline]

http://ucce.ucdavis.edu/datastore/datastoreview/showpage.cfm?usernumber=146&surveynumber=351

Micro-shoot tip tissue culture is the method of choice to eliminate viruses and other pathogens from many plant species. The micro-shoot tip culture technique has the advantage is regenerating a single plant from a single, minuscule (approximately 0.5 mm) shoot. The technique also avoids the production of plants from callus which can lead to regeneration of an off plant. The combination of low hormone levels combined with a minimum time in culture reduces the chance of mutation and regeneration of an off-type plant.

 

[accessndx]

Pipeline, you bring up a good point. However, people are going to create different phenotypic expressions of the same genome merely by the differences in environment, nutrients and overall care. I'm not certain that the risk of epigentic drift is a significant enough barrier to disregard the potential for tissue culture to be used as storage.

 

[still]

bump.......