Cloning dead plant material?

[Perpetual]

So...had a crazy idea, and wanted to hear others thoughts.

I have dead, dried buds.

I want to extract the DNA from the material, and insert that DNA into a new seed, hoping for something like the original.

Is this possible?

No matter the cost or difficulty, what would be involved? Anyone a genetics wiz?

 

[resin_lung]

It's never a simple question with you, is it Perpetual! LOL

 

[geopolitical]

Completely dead? Probably not into a new plant with existing tech.

http://www.newscientist.com/article/mg18324630.400-cloning-the-dead.html

Interesting though, no?

 

[Perpetual]

Well resin_lung, it has been bouncing around in my head for a while now. I'm just not sure who to turn to on this! One person told me it was possible, but I would need 100's of thousands of dollars in equipment. I was thinkin' there might be a pot-friendly student who has some fancy lab access? I'd be so down to go for it if it held any promise. And, if there were a way around the 100's of thousands of dollars!

Certainly an interesting article, geopolitical. Kind of freaky mixing dead children cells with fresh cow cells!

 

[hempknowsis]

Monsanto could probably accomplish this.

 

[indica_maniac]

no offense, I know u were just kidding but FUCK MONSANTO! those bastards are ruining the world one seed at a time. Or should i say one gene at a time lol

 

[geopolitical]

Monsanto could probably accomplish this.

Monsanto can't even manage to properly engineer termination seeds . . .

 

[JWH-018]

It's possible to grow certain plants from single cells currently, and it's possible to introduce specific foreign genes into live plants but the process is significantly more difficult and time consuming than cloning processes are for animals. Plants generally have significantly larger amounts of chromosomal material, grow very slowly and have a very few cloning vectors that can successfully function in plant cells. But there are papers out there about some of the easier plants to clone such as carrots, tomatoes, potatoes, petunias, and cabbage.

I'm sure I don't have to say that this is all being done by very well trained teams of scientists with many millions in funding and operating in high tech labs at universities and commercial biogenics labs. You won't be cloning your bag weed with an ez cloner (single cell edition) in your basement within our lifetimes. If you are you should stop growing weed for money and get a real job, you'd be paid much better.

EDIT:

Monsanto can't even manage to properly engineer termination seeds . . .

I know. It sucks we have to rely on skynet to send them back in time still.

 

[lysol]

Jurassic park?

And if we can alter specific genes would it then be possible / and possibly more cost effective to try to produce a new synthesized strain that has similar characteristics to the dried bud? As it is we can only measure like 5% of the active chemicals in there tho anyways

 

[h^2 O]

depends on whether there's any DNA material or living cells left. If you could extract DNA and culture it - theoretically it would culture into an embryo? No..wait. Replicate it? No. Pretty sure no. But you could extract DNA then somehow do something under the microscope...like take pollen and the cells you have and artifically inseminate a make-shift seed? Weird question. I think of course you could clone it if it's dead...just need to extract its DNA. Depends if there's still sufficient DNA in the buds. We have the technology to clone humans and extinct animals (which we probably have done), so don't see why not

 

[r13f]

could be an interesting field of study, but who is holding onto the research grant dough?

 

[GhostToker]

It can defnately be done but it would cost an absolute fortune in equipment, and you'd need a lab. You cant insert DNA into a seed, the seed is allready a complete specimen. You'd need to intercept a newly fertilized bud cell where the seed will be formed and insert the dna into that.. it would basically be extraordinarily complicated and expensive, but not impossible

 

[h^2 O]

could be an interesting field of study, but who is holding onto the research grant dough?

The DuPont Corporation's lobbyists

 

[Perpetual]

It's possible to grow certain plants from single cells currently, and it's possible to introduce specific foreign genes into live plants but the process is significantly more difficult and time consuming than cloning processes are for animals. Plants generally have significantly larger amounts of chromosomal material, grow very slowly and have a very few cloning vectors that can successfully function in plant cells. But there are papers out there about some of the easier plants to clone such as carrots, tomatoes, potatoes, petunias, and cabbage.

I'm sure I don't have to say that this is all being done by very well trained teams of scientists with many millions in funding and operating in high tech labs at universities and commercial biogenics labs. You won't be cloning your bag weed with an ez cloner (single cell edition) in your basement within our lifetimes. If you are you should stop growing weed for money and get a real job, you'd be paid much better.

EDIT:


I know. It sucks we have to rely on skynet to send them back in time still.

Excellent, thanks for ideas one where to read up on some things.

I understand this is quite a task. I am just feeling it out, and quite curious. An old horticultural teacher I asked about this advised me of the very, very expensive things needed. Well, not so specifically. I didn't ask him about the equipment. But, funding an experiment in an existing lab is something I'd consider, if it were there as an option.

What does anyone think that might cost?

 

[junior_grower]

I have access to the labs needed. But even as a student with full access I need paper work in place for every machine I use. last term some of the Bio eng projects ( genetic modification of hops) had a budget of 1.5 million just to help improve the mold resistance. In order to begin cloning in the way we are talking about you would be in the millions of dollars to fund such and endeavor.

 

[JWH-018]

Well, I'm talking totally out of my ass here as I don't have the background, but I'd guess you're talking mid seven figures minimum just to get in the door. I did some work at a place six years ago that was setting up a small (their words) GMO lab, and the capital budget alone was 25 million and I believe they went well over. Just the IT portion was nearly 2M. I don't know how much of that transfers, but its something. I'd think staffing would be particularly hard at least in the US because of the possible drawbacks of being shut out of government funded projects and the relatively low scientific value of your experiment.

Things like this are more typically done by providing a grant to a suitable university lab but you'd need to find an appropriate venue - in the states the DEA would prevent them from using your own cannabis I believe. Once you hit up a good location I think the whole process would end up being a negotiation based on physical costs as well as how busy they are, political climate, who gets any patents, how valuable they might be and how much scientific value/publishable the work would be. I think you'd be on the wrong side of at least 3 of those issues, again I'm going to bald ass guess a few mill but you'd never know until you asked a specific institution.

 

[Perpetual]

Hmm. Those are indeed large amounts of capital. Help me to understand why the particular project I had in mind would require that amount? Anyone have an of the names of the equipment necessary? Is it something that requires a lot of labor or 100's of trials and errors, is it quite difficult to lock down the needed material to add to the new plant cell? Or is this a relatively straight forward process, something that is just a step by step thing that has been done before?