Plant tissue culture?

[maryj315]

Many different plants are successfully propagated by tissue culture such as ferns, Syngoniums, bananas, tobacco, orchids, and African violet. The Plant Tissue Kit provides the materials to culture most common plants by shoot multiplication or generation of shoots from different plant tissues. That is the magic of plant tissue culture. You can make new plants grow from pieces of leaves, stems, flowers, or roots, just about anything!

Universities and research companies maintain expensive laboratories for multiplying their valuable plants and exposing their hidden genetics. The Super Starts micropropagation kit provides the tools and information to successfully do plant tissue culture at home.

The plants become self-multiplying clusters that yield hundreds of new plants every few weeks, eliminating mother plants. The biggest plants are removed for rooting and the rest will go on to multiply and grow. The new plants are removed and planted in your favorite soil. Soon their roots are established and the new plants explode with new bushy growth energized by the sugar rush and hormone influence of the tissue culture media. Imagine a clone 5 inches tall with miniature leaves and 6-8 branches, a real bonsai that grows to beat any seed or cloned plant.

Any plant person who can make gelatin and handle tweezers can take advantage of plant tissue culture. Using plant tissue culture to multiply plants and make super starts is called micropropagation. Micropropagation feeds sugar, nutrients, and hormones to tissues of your favorite plants, making them multiply like crazy. Instead of taking cuttings from mother plants that require lots of space and time, you can multiply plants in glass jars and tubes under a small fluorescent light. The jars don’t wear out like mother plants and regularly make many new plants every couple of weeks.



Thought i would edit it to give the information they are giving this is a ad for a home kit at Texas hydroponics just wanted to see if it was even practical for our use.

 

[C21H30O2]

it is practical for breeders but not really for anyone else. tissue culture clones will take longer to develop as they are only starting from a few cells. but it is an excellent way to keep many mothers (1000s would be easily stored in a freezer) for an extended period of time. dont be afraid to try it though if you have a passion for it. just make sure to start a thread and keep us up to date.

 

[maryj315]

So what kind of set up our we looking at here? would a small cab do the trick? they make it sound pretty simple. i mean a never ending supply of clones with out the need for mothers sounds good but not if it takes to much space or time. so has anybody tried this before?

 

[afungi]

You would need, a laminar flow hood with a 99.999% efficiency. An autoclave, petri dishes, test tubes, baby food jars. Then all the media nutrients and a scale to weigh out amounts. Plus tweezers, scalpel, alcohol lamp, and a flask for cooking media.

Fighting the war on germs in never easy. With sound practiced and a sterile technique one can lower the losses and up the production.

But imagine that it only takes one sneeze or cough, one flake of dandruff, one spore to land on and inoculate the culture media and you are screwed.

Start growing mushrooms with a lab. Start mixing media, like pda or mda. Maybe make some liquid cultures too. Start selecting and isolating substrains. Using a sterile technique. Learn how to dissect a culture and move it smoothly to other containers. The quicker and smoother you can do something the less risk of contamination.
Open a pitri dish in a room in the house for a split second, let it sit a week to incubate and check out the zoo you started.

Just saying it's a lot to cover, mushroom cultivation would give you more basics that you can use with the more advanced techniques that you would use with plant tissue culture.

I think instead of going to callus culture, I'd look more into shoot replication. Take a micro cutting move that to rooting media, take that cutting move it to a shoot replication media. Take the shoots and move them to rooting media. Take those and move them to shoot replicating media. You could take a plant. Chop each node and plant them in rooting media. You could get 100 micro cuttings from a nice bush. You could grow them out in baby food jars then take more cuttings from the original cuttings.

In theory it's a great way to multiply a specimen.

Like with mushrooms and grain to grain. One jar of spawn can inoculate 10 jars. Those ten jars could inoculate 100 jars. the 100 jars then becomes 1000 jars.

 

[kaljukajakas]

http://www.omnisterra.com/botany/cp/slides/tc/tc.htm

It's quite possible to do tissue culture at home without much specialized stuff. You can use a pressure cooker for sterilizing instead of an autoclave and you can easily get away with not using a laminar flow hood. You could even make your own sterile workspace by simply taking a small cabinet and gluing a piece of plexiglass in front of it so there's room for your hands at the bottom and then just blowing air into it from the top through a good HEPA filter.

Proper technique is important but then again I once licked and coughed on a LB-ampicillin plate and then left it to incubate for a week: nothing grew on it. One can often use antibiotics for tissue culture too...

 

[accessndx]

I'm most interested in the ability to store a plethora of different strains in culture form indefinitely in a cold storage unit. I'm only now starting to investigate this "tissue culture" information, so I'll ask the generic question with the hope someone can point me in the right direction....
How long are these cultures viable for when stored in cold?
How much work is involved in the storage of the cultures?

I see that the micropropagation itself consists of numerous steps to get the culture to become a viable and rooted plant. But to take strains and just preserve them for cryostasis should be markedly easier....
What are your thoughts? Has anyone had any practical experience with this?

 

[Hydro-Soil]

How 'transportable' are these cultures?

Would they easily survive a mail trip across an ocean or two?

 

[drow]

I would imagine transporting them would require little or no effort and getting pulled over with them wouldnt really bat an eye if you cleverly disguised it. Interesting, would love the idea to be able to store 100s of strains

 

[C21H30O2]

If you can make cannabis tissue culture you should be able to make artificial seeds which make transport about as easy as transporting regular seeds.

 

[Hydro-Soil]

If you can make cannabis tissue culture you should be able to make artificial seeds which make transport about as easy as transporting regular seeds.

Seeds aren't exact though. I already have access to seeds. No thanks, if I can avoid it.

What I want is to be able to take a sample of a plant anywhere in the world and be able to grow it in MY house. Seeds won't give me the 'exact' plant without a crapload of growing out seeds and testing and looking and BLEAH! That's dumb.

If you want something 'close' seeds are great and I have lots of those plants but seed are no substitute for a clone of the real thing.

I don't think you mean 'artificial' seeds either. Have researchers figured out how to make a synthetic seed yet?

 

[accessndx]

Yeah, researchers have been able to develop "artificial seeds". I've seen some pictures here and there...and I know that I saw a whole write up on them (maybe even in Wikipedia?). I was uncertain what they were in fact...however it appears to make use of this tissue culture technique...and what you receive is essentially a micronized cut that is suspended in some sort of nutritive mix....and shaped like a round bead.
Pretty neat stuff.
I'd still like to get some solid information on how long these cultures are viable for?
Can I keep them in the freezer for 10 years? Somehow, I think alot of misinformation is being distributed...and there's alot of speculation.
It doesn't seem realistic to keep living tissue on ice without detriment. I know that ice crystals form when freezing tissue. Doesn't that put the kibosh on the whole idea of keeping this stuff around on lockdown?
If you look on youtube for micropropagation, there's a guy that does an at home tutorial. The first 3 are kinda B.S. Sesame Street stuff, but you can see he's got like hundreds of little baby food jars sitting all over the place...with what looks like tiny little plants everywhere. I didn't see any cryogenic stasis units, no freezers, etc.
It looked essentially like alot of cloning, but from small tiny little pieces of plant.
That's great and all, but I don't need another method to clone. I need a way of putting strains in deep storage so I can feel free to run others. If I am craving something from the vault, I can break it out, thaw it...work it up and have a new momma in a bit of time.
That's basically all I'm looking for.
Of course if you can do all that, you can certainly send stuff in the mail, bring things on planes...travel freely, etc. That's fairly obvious.
Does anyone have any real world experience with this stuff?

 

[C21H30O2]

Seeds aren't exact though. I already have access to seeds. No thanks, if I can avoid it.

What I want is to be able to take a sample of a plant anywhere in the world and be able to grow it in MY house. Seeds won't give me the 'exact' plant without a crapload of growing out seeds and testing and looking and BLEAH! That's dumb.

If you want something 'close' seeds are great and I have lots of those plants but seed are no substitute for a clone of the real thing.

I don't think you mean 'artificial' seeds either. Have researchers figured out how to make a synthetic seed yet?

yes i do mean artificial. they are artificial seeds that means they have a tissue culture inside of them ie a clone so yes you can get the exact same plant. http://www.sp.edu.sg/schools/cls/bioline_08.htm

 

[C21H30O2]

Yeah, researchers have been able to develop "artificial seeds". I've seen some pictures here and there...and I know that I saw a whole write up on them (maybe even in Wikipedia?). I was uncertain what they were in fact...however it appears to make use of this tissue culture technique...and what you receive is essentially a micronized cut that is suspended in some sort of nutritive mix....and shaped like a round bead.
Pretty neat stuff.
I'd still like to get some solid information on how long these cultures are viable for?
Can I keep them in the freezer for 10 years? Somehow, I think alot of misinformation is being distributed...and there's alot of speculation.
It doesn't seem realistic to keep living tissue on ice without detriment. I know that ice crystals form when freezing tissue. Doesn't that put the kibosh on the whole idea of keeping this stuff around on lockdown?
If you look on youtube for micropropagation, there's a guy that does an at home tutorial. The first 3 are kinda B.S. Sesame Street stuff, but you can see he's got like hundreds of little baby food jars sitting all over the place...with what looks like tiny little plants everywhere. I didn't see any cryogenic stasis units, no freezers, etc.
It looked essentially like alot of cloning, but from small tiny little pieces of plant.
That's great and all, but I don't need another method to clone. I need a way of putting strains in deep storage so I can feel free to run others. If I am craving something from the vault, I can break it out, thaw it...work it up and have a new momma in a bit of time.
That's basically all I'm looking for.
Of course if you can do all that, you can certainly send stuff in the mail, bring things on planes...travel freely, etc. That's fairly obvious.
Does anyone have any real world experience with this stuff?

artificial seeds can stay viable for years if stored properly. That means not a freezer but a refrigerator that keeps the seeds slightly above freezing so as to slow their metabolism to a crawl. A typical refrigerator can hold 1000's if not 10s of thousands of different strains.

 

[Hydro-Soil]

artificial seeds can stay viable for years if stored properly. That means not a freezer but a refrigerator that keeps the seeds slightly above freezing so as to slow their metabolism to a crawl. A typical refrigerator can hold 1000's if not 10s of thousands of different strains.

Sweet, ya learn something new every day.

 

[sunnydog]

You would need, a laminar flow hood with a 99.999% efficiency. An autoclave, petri dishes, test tubes, baby food jars. Then all the media nutrients and a scale to weigh out amounts. Plus tweezers, scalpel, alcohol lamp, and a flask for cooking media.

Fighting the war on germs in never easy. With sound practiced and a sterile technique one can lower the losses and up the production.

But imagine that it only takes one sneeze or cough, one flake of dandruff, one spore to land on and inoculate the culture media and you are screwed.

Start growing mushrooms with a lab. Start mixing media, like pda or mda. Maybe make some liquid cultures too. Start selecting and isolating substrains. Using a sterile technique. Learn how to dissect a culture and move it smoothly to other containers. The quicker and smoother you can do something the less risk of contamination.
Open a pitri dish in a room in the house for a split second, let it sit a week to incubate and check out the zoo you started.

Just saying it's a lot to cover, mushroom cultivation would give you more basics that you can use with the more advanced techniques that you would use with plant tissue culture.

I think instead of going to callus culture, I'd look more into shoot replication. Take a micro cutting move that to rooting media, take that cutting move it to a shoot replication media. Take the shoots and move them to rooting media. Take those and move them to shoot replicating media. You could take a plant. Chop each node and plant them in rooting media. You could get 100 micro cuttings from a nice bush. You could grow them out in baby food jars then take more cuttings from the original cuttings.

In theory it's a great way to multiply a specimen.

Like with mushrooms and grain to grain. One jar of spawn can inoculate 10 jars. Those ten jars could inoculate 100 jars. the 100 jars then becomes 1000 jars.

Fortunately We do not need to isolate single strains anymore, that was the old days!
I saw this kit at my grow shop, best use I can see would be to ship genetic stock.
Takes a long time to propagate, and like afungi said, there is a risk of contamination (they fail to mention this in their promo materials).
Used to have a laminar flow hood hood, back in the day.
If you keep your shit clean, and work fast, you can get away without it,(I Have).

SD

 

[Indica Jones]

I already clone shrooms, and it is necessary to keep the air scrubbed. I think Breeder Steve was talking about going in this direction instead of using seed. Didn't hear him talk about it again though.

 

[Hydro-Soil]

Fortunately We do not need to isolate single strains anymore, that was the old days!
I saw this kit at my grow shop, best use I can see would be to ship genetic stock.
Takes a long time to propagate, and like afungi said, there is a risk of contamination (they fail to mention this in their promo materials).
Used to have a laminar flow hood hood, back in the day.
If you keep your shit clean, and work fast, you can get away without it,(I Have).

SD

I've used the old 'Oven Tek' instead of a laminar flow hood. Not all ovens work that well but sure worked like magic for me.

You simply work with your cultures while they're sitting on the oven rack pulled most of the way out of the oven. Leave the oven on the lowest setting.
The rising heated air keeps a fresh flow of air going past the jar without contaminants.

Sterility is just a process. You can set up a sterile environment in any room that can be sealed against drafts.

 

[Indica Jones]

Or in a rubbermaid box with sealed gloves and a clear window.

 

[dongle69]

Greenhouse is supposed to be coming out with the artificial seeds.
Should be fun.

 

[Hydro-Soil]

Greenhouse is supposed to be coming out with the artificial seeds.
Should be fun.

I'm not too impressed with GH these days.


I've done a bit of reading and it seems that each type of plant requires work to create the right type of material makeup for the artificial seed coating.

I don't find anything on anyone posting that kind of info though.

I'll stick to cultures then.