GMO Rules! Brew your own drugs.

[Rider420]

Researchers from Stanford University, with funding from NCCIH, have created an innovative way to produce opioid drugs from sugar using genetically modified yeast.

Genetically Modified Yeast Strains Produce Opioid Drugs From Sugar

Researchers from Stanford University, with funding from NCCIH, have created an innovative way to produce opioid drugs from sugar using genetically modified yeast. The research is described in a recent issue of the journal Science.

www.nccih.nih.gov

Scientists prove that psilocybin, a potential drug for treating depression and other psychological conditions can be produced in yeast.

Psychedelic compound from magic mushrooms produced in yeast

Scientists prove that psilocybin, a potential drug for treating depression and other psychological conditions can be produced in yeast.

www.sciencedaily.com

Using genetic modification, researchers have reprogrammed a relative of a tobacco plant to produce cocaine in its leaves.

Genetically modified tobacco plant produces cocaine in its leaves

Researchers have reproduced the entire biochemical pathway for how coca plants make cocaine in another plant, which could help people manufacture the drug for scientific study

www.newscientist.com

 

[Creeperpark]

Finally, we can talk about this stuff out loud without worrying about talking too loud. I'm ready for some good old tripping days to come back. Maybe now we won't have to be so freaking paranoid all the time when tripping.

 

[Microbeman]

Researchers from Stanford University, with funding from NCCIH, have created an innovative way to produce opioid drugs from sugar using genetically modified yeast.

Genetically Modified Yeast Strains Produce Opioid Drugs From Sugar

Researchers from Stanford University, with funding from NCCIH, have created an innovative way to produce opioid drugs from sugar using genetically modified yeast. The research is described in a recent issue of the journal Science.

www.nccih.nih.gov

Scientists prove that psilocybin, a potential drug for treating depression and other psychological conditions can be produced in yeast.

Psychedelic compound from magic mushrooms produced in yeast

Scientists prove that psilocybin, a potential drug for treating depression and other psychological conditions can be produced in yeast.

www.sciencedaily.com

Using genetic modification, researchers have reprogrammed a relative of a tobacco plant to produce cocaine in its leaves.

Genetically modified tobacco plant produces cocaine in its leaves

Researchers have reproduced the entire biochemical pathway for how coca plants make cocaine in another plant, which could help people manufacture the drug for scientific study

www.newscientist.com

I'm not sure I get the opioid one. Production of opioids in the lab is old news; methadone, oxycodone, tramadol, darvon, demoral, etc. etc. Opiates on the other hand are derived from the poppy; opium, morphine, heroin, codeine. The authors seemingly don't know what they are talking about.

 

[St. Phatty]

I'm not sure I get the opioid one. Production of opioids in the lab is old news; methadone, oxycodone, tramadol, darvon, demoral, etc. etc. Opiates on the other hand are derived from the poppy; opium, morphine, heroin, codeine. The authors seemingly don't know what they are talking about.

Hard to patent the drugs that come from the poppy.

Maybe that's what they're scared of.

 

[Rider420]

Finally, we can talk about this stuff out loud without worrying about talking too loud. I'm ready for some good old tripping days to come back. Maybe now we won't have to be so freaking paranoid all the time when tripping.

Activist Dana Larsen opens new Vancouver store selling psilocybin mushrooms, LSD and more - BC News

Dana Larsen is on a mission to provide Metro Vancouverites with a safe supply of illicit substances -- but he's doing it in phases.

www.castanet.net

​

Activist Dana Larsen opens new Vancouver store selling psilocybin mushrooms, LSD and more​

Dana Larsen is on a mission to provide Metro Vancouverites with a safe supply of illicit substances -- but he's doing it in phases.

"I have not taken all the mushrooms on the menu," he notes, describing how he has a dozen "shroom" strains for sale at his new shop.

"I do microdose. I take LSD sometimes; I take mushrooms sometimes...I try to sample what I can but I have not tried every product in the store."

While he hopes to open a brick-and-mortar compassion club for heroin users in the future, the advocate currently offers a host of psychedelic drugs and what he describes as "plant-based medicine."

The new shop opened at 247 W Broadway on Monday, Feb. 13, and offers a range of illegal products. Psilocybin mushrooms, more commonly referred to as "magic mushrooms," are some of the most popular items for sale. Each one of them offers a distinct experience, ranging from a more relaxing sensation to a profoundly psychedelic journey, Larsen says.

The hallucinogenic fungi can also be micro-dosed for more manageable "trips," he says.

When people buy from mushroom dispensaries, they are made aware of the dose they are taking. They also know that they are getting a "safe supply" that has been tested rather than purchasing their drugs on the street, according to Larsen.

The Vancouver advocate founded a community drug-testing service called Get Your Drugs Tested in 2019, which offers a place for drug users to get their substances checked for harmful contaminants, seven days a week, free of charge.

"We've tested over 40,000 substances now," he remarks, adding that all of the results are on a searchable database on the website. "We're doing like two-thirds of all the drug testing in the province from our one location at 880 East Hastings."

Magic mushrooms Vancouver store opens on Broadway​

Customers can also purchase LSD in a diluted liquid form where they can mircodose it or "take it for psychedelic purposes." An LSD trip lasts significantly longer than an average magic mushroom trip, notes Larsen, with the effects of the former lasting upwards of 10 hours. Both substances are included under Schedule III of the Controlled Drugs and Substances Act of the Criminal Code.





Larsen also sells DMT and Peyote, which are derived from plants with psychoactive qualities, as well as coca leaf tea -- an herbal tea that is derived from the same plant that cocaine comes from.

Kratom, another item on the menu, comes from a tree in southeast Asia and is used as a substitute for street opiates and fentanyl. While it is not in the controlled substances act, it is not to be sold for "human consumption."

While many advocates have touted the therapeutic benefits of using magic mushrooms, Health Canada cautions that they can cause people to have intense fear and paranoia, possibly resulting in "bad trips" or "flashbacks. These situations may "lead to risk-taking behaviour, which may then lead to traumatic injuries or even death."

But Larsen believs the benefits of legalizing substances like Psilocybin far outweigh the cost of lives lost and people putting themselves in harm's way to access them.

"I hope that this is the beginning of more stores like this in Vancouver," he says. "This is part of an effort to end the war on drugs by trying to do civil disobedience.

"I don't think we would have legalized marijuana in Canada if there wasn't a massive act of civil disobedience from coast to coast."

 

[Rider420]

I'm not sure I get the opioid one. Production of opioids in the lab is old news; methadone, oxycodone, tramadol, darvon, demoral, etc. etc. Opiates on the other hand are derived from the poppy; opium, morphine, heroin, codeine. The authors seemingly don't know what they are talking about.

LOL I love growing cannabis and mushrooms as well as brewing beer wine and now something new opioids AT HOME!

 

[St. Phatty]

Activist Dana Larsen opens new Vancouver store selling psilocybin mushrooms, LSD and more - BC News

Dana Larsen is on a mission to provide Metro Vancouverites with a safe supply of illicit substances -- but he's doing it in phases.

www.castanet.net

​

Activist Dana Larsen opens new Vancouver store selling psilocybin mushrooms, LSD and more​

I would like to see them open a store with a bunch of CAD workstations AND the Stimulants for Sale.

 

[Rider420]

I would like to see them open a store with a bunch of CAD workstations AND the Stimulants for Sale.

Just take your laptop to your local coffee shop buddy.

 

[Microbeman]

Activist Dana Larsen opens new Vancouver store selling psilocybin mushrooms, LSD and more - BC News

Dana Larsen is on a mission to provide Metro Vancouverites with a safe supply of illicit substances -- but he's doing it in phases.

www.castanet.net

​

Activist Dana Larsen opens new Vancouver store selling psilocybin mushrooms, LSD and more​

Dana Larsen is on a mission to provide Metro Vancouverites with a safe supply of illicit substances -- but he's doing it in phases.

"I have not taken all the mushrooms on the menu," he notes, describing how he has a dozen "shroom" strains for sale at his new shop.

"I do microdose. I take LSD sometimes; I take mushrooms sometimes...I try to sample what I can but I have not tried every product in the store."

While he hopes to open a brick-and-mortar compassion club for heroin users in the future, the advocate currently offers a host of psychedelic drugs and what he describes as "plant-based medicine."

The new shop opened at 247 W Broadway on Monday, Feb. 13, and offers a range of illegal products. Psilocybin mushrooms, more commonly referred to as "magic mushrooms," are some of the most popular items for sale. Each one of them offers a distinct experience, ranging from a more relaxing sensation to a profoundly psychedelic journey, Larsen says.

The hallucinogenic fungi can also be micro-dosed for more manageable "trips," he says.

When people buy from mushroom dispensaries, they are made aware of the dose they are taking. They also know that they are getting a "safe supply" that has been tested rather than purchasing their drugs on the street, according to Larsen.

The Vancouver advocate founded a community drug-testing service called Get Your Drugs Tested in 2019, which offers a place for drug users to get their substances checked for harmful contaminants, seven days a week, free of charge.

"We've tested over 40,000 substances now," he remarks, adding that all of the results are on a searchable database on the website. "We're doing like two-thirds of all the drug testing in the province from our one location at 880 East Hastings."

Magic mushrooms Vancouver store opens on Broadway​

Customers can also purchase LSD in a diluted liquid form where they can mircodose it or "take it for psychedelic purposes." An LSD trip lasts significantly longer than an average magic mushroom trip, notes Larsen, with the effects of the former lasting upwards of 10 hours. Both substances are included under Schedule III of the Controlled Drugs and Substances Act of the Criminal Code.





Larsen also sells DMT and Peyote, which are derived from plants with psychoactive qualities, as well as coca leaf tea -- an herbal tea that is derived from the same plant that cocaine comes from.

Kratom, another item on the menu, comes from a tree in southeast Asia and is used as a substitute for street opiates and fentanyl. While it is not in the controlled substances act, it is not to be sold for "human consumption."

While many advocates have touted the therapeutic benefits of using magic mushrooms, Health Canada cautions that they can cause people to have intense fear and paranoia, possibly resulting in "bad trips" or "flashbacks. These situations may "lead to risk-taking behaviour, which may then lead to traumatic injuries or even death."

But Larsen believs the benefits of legalizing substances like Psilocybin far outweigh the cost of lives lost and people putting themselves in harm's way to access them.

"I hope that this is the beginning of more stores like this in Vancouver," he says. "This is part of an effort to end the war on drugs by trying to do civil disobedience.

"I don't think we would have legalized marijuana in Canada if there wasn't a massive act of civil disobedience from coast to coast."

Kinda cool, except for peyote, which is endangered. Kratom is absolute shit. It would be great if he sold poppy pods. Those are the real deal.
The downtown eastside - where I grew up - attended college at the Anchor Hotel with Harold Hedd and the rest of the gang.

 

[Microbeman]

LOL I love growing cannabis and mushrooms as well as brewing beer wine and now something new opioids AT HOME!

Tell us when you master the 20 steps involved.

 

[St. Phatty]

Kinda cool, except for peyote, which is endangered. Kratom is absolute shit. It would be great if he sold poppy pods. Those are the real deal.
The downtown eastside - where I grew up - attended college at the Anchor Hotel with Harold Hedd and the rest of the gang.

The DEA has a *.pdf about poppy seeds that spells it out.

Raw poppy seeds are coated in Morphine and Codeine - in very small amounts.

No refinement needed !

There are a lot of Opium experiences on Erowid & Hyperreal, from opium tea, made with pods.

 

[buzzmobile]

The DEA has a *.pdf about poppy seeds that spells it out.

Raw poppy seeds are coated in Morphine and Codeine - in very small amounts.

No refinement needed !

There are a lot of Opium experiences on Erowid & Hyperreal, from opium tea, made with pods.

Service Members Should Avoid Foods With Poppy Seeds

It's not an urban legend: Eating poppy seeds can cause diners to test positive for codeine on a urinalysis, Defense Department officials said in a memo.

www.defense.gov

 

[Rider420]

LOL

Tell us when you master the 20 steps involved.

To make morphine or heroin? Becuase you get the same opium from the GMO yeast as you get from the sap of poppies. FYI your better off drinking then smoking.

What is the Difference Between Opium vs Heroin? - Crest View

Learn about the difference between opium vs heroin, and the treatment options for addiction at Crest View Recovery Center in Asheville, NC.

www.crestviewrecoverycenter.com

So is opium heroin? Heroin is a synthetic substance that uses opium as its primary active ingredient. The manufacturing process dictates manufacturers convert the opium into pure morphine, which then turns into chemically-changed heroin. The process produces a substance that has a higher level of potency than the opium extract. The higher potency leads to more euphoria and eventually, more significant problems.

 

[Microbeman]

To make morphine or heroin? Becuase you get the same opium from the GMO yeast as you get from the sap of poppies.

I believe the article stated that there are 20 steps involved in producing the opioid from yeast. Making morphine and heroin is simple and as stated they are 'opiates', derived from poppies. Morphine (from Morpheus, the god of sleep) is a natural alkaloid contained in the poppy head. Making a tea from the powdered pods is every bit as good as processed morphine or heroin. I first had morphine about the age of 1 and have used it regularly since 95 as an analgesic. The other molecular structures I mentioned in my earlier post are synthetic 'opioids' which are not derived from the poppy plant but manufactured by copying the morphine and enkephalin structure. I may be wrong but believe this is what the scientists are doing with the yeast/sugar.

 

[Rider420]

'Home-brew' morphine from brewer's yeast now possible -study

www.consultant360.com

Scientists, however, recently reported engineering yeast to carry out the second part of the 15-step opioid-producing reaction. What remained was just the hurdle of coaxing yeast to carry out the first part.

That is what scientists, led by Dr. John Dueber of the University of California, Berkeley, have accomplished.

They isolated a crucial enzyme from sugar beets, mutated its gene to make it more productive, and slipped it into yeast.

They then added more foreign DNA and achieved their goal: the yeast carried out the first half of the reaction that produces opioids.

Combined with the earlier recipe for the rest of the reaction, said Dr. Dueber, "it's now a matter of linking (the steps) together and scaling up the process."

Outside scientists agreed, and expressed alarm over that prospect.

The process described in Nature Chemical Biology is inefficient, requiring 300 liters of genetically engineered yeast to produce a single 30 milligram dose of morphine.

But with improvements that are well within reach, that dose could be obtained from "a glass of yeast culture grown with sugar on a windowsill," Dr. Voight said.

Note this was done in 2015 eight years ago! And opioid users are everywhere lawyers judges and yes even scientists.

 

[Microbeman]

 

[Microbeman]

 

[Microbeman]

The E. coli opiate production system developed in this study does not require specific substrates and resulted in 2.1 mg l−1 thebaine production, which is a 300-fold improvement compared with the reported yeast system8. This large improvement is associated with high enzyme activity in E. coli. In the yeast system, thebaine titres are ∼0.2 μM from 1 mM (R,S)-THP; the conversion efficiency is typically only 0.02% (ref. 8). However, in the E. coli system, the conversion efficiency of thebaine from (R,S)-THP was 12.5% (6.8 μM thebaine was produced from 54.5 μM (R,S)-THP diluted via stepwise culture). As the (R,S)-reticuline production system in yeast exhibits a 10% conversion efficiency of (R,S)-reticuline from (R,S)-THP34, the large difference in thebaine productivity between E. coli and yeast can be attributed to the activity of SalS, SalR and SalAT, which are required for thebaine synthesis from (R)-reticuline. In another yeast system, the thebaine conversion efficiency from (R)-reticuline is only ∼1% (ref. 7). The conversion efficiency of thebaine from biosynthesized (R)-reticuline reached 80% in the E. coli system, indicating that the activity of SalS, SalR and SalAT was much stronger in E. coli than in yeast. This strong activity may be related to high expression of these enzymes or high productivity of co-factor(s), for example, haeme, NADPH and/or acetyl-CoA in E. coli. These results demonstrated that E. coli is suitable for opiate production, despite concerns related to the difficulty in expressing plant genes in the species13,34.

Despite the high conversion efficiency of thebaine from (R)-reticuline, the production yield of thebaine was relatively low in this total biosynthesis system. These results suggested that the productivity of (R)-reticuline is a major rate-limiting step. To develop a practical production system for opioids, increasing the productivity of (R)-reticuline is the greatest challenge. We demonstrated two ways to produce (R)-reticuline, that is, using STORR or the irregular activity of a methyltransferase. Although the conversion of racemic THP via methyltransferases was used to reconstruct opiate biosynthesis in this study, half of the reticuline that was produced, (S)-reticuline, was not used for opiate production. On the other hand, STORR has low activity in E. coli, even after the addition of an expensive cofactor for (R)-reticuline production. To improve (R)-reticuline productivity, these drawbacks should be overcome by metabolic engineering strategies such as protein engineering for practical production.

Engineered yeast can convert thebaine into various opiates such as morphine33. Theoretically, other opioids in addition to hydrocodone can be produced by integrating opioid biosynthetic enzymes such as codeinone reductase (COR), codeine demethylase and morphine dehydrogenase into our thebaine total biosynthesis system. Alternatively, a hybrid E. coli-yeast system could enable opioid production35. Thus, it is possible, in principle, to produce various opioids from a simple carbon source using microbes.

Stepwise culture has some advantages over single-step culture in the thebaine production system. Because tyrosinase degrades THP, dividing the THP production pathway into two parts enables efficient THP production12. Thus, stepwise culture bypasses undesirable reactions in which upstream enzymes act on downstream compounds. The other benefit of stepwise culture is that it permits the individual optimization of each culture step, like chemical synthesis methods. In this study, IPTG had a positive effect on thebaine production, although it diminished (R)-reticuline production (Supplementary Figs 5 and 6). Accordingly, dividing the thebaine synthetic pathway from (R,S)-THP into two parts via stepwise culture enabled successful opiate production. However, it was not clear why IPTG had the opposite effect. To construct a practical production system, we aim to verify the necessity of separating this step by elucidating the exact mechanism that does not allow the production of thebaine with (R,S)-reticuline production in our future research.

Recently, microbial production of plant secondary metabolites has attracted attention5,6,8,13,36,37. These compounds require multistep reactions from a simple carbon source, and are associated with undesirable reactions and enzyme inhibition. The efficient production of these compounds may be achieved using stepwise culture, which would minimize problems that arise in multi-step reactions. Furthermore, a stepwise culture method enables the segmentation of a synthetic pathway, and that facilitates the production of similar compounds. In fact, the central intermediate in benzylisoquinoline alkaloid biosynthesis, (S)-reticuline, is used for the production of protoberberine with the berberine bridge enzyme, scoulerine O-methyltransferase and CYP719A1 (ref. 38). Similarly, aporphine alkaloids are produced from (S)-reticuline with CYP80G2 and CNMT enzymes35, whereas benzophenanthridine alkaloids are produced via scoulerine with CYP719A5, CYP719A3, TNMT, MSH and P6H39. By constructing these enzyme expression strains, and using an (S)-reticuline production step, a stepwise culture method can enable the production of various kinds of alkaloids. Thus, stepwise culture methods enable flexibility in the production of various compounds, similar to conventional chemical synthesis methods. The E. coli system developed in this study thus represents a potentially useful platform for the further development aimed at the industrial production of opiates.

Total biosynthesis of opiates by stepwise fermentation using engineered Escherichia coli - Nature Communications

Opiates—the gold standard for pain relief—are currently produced by extraction from opium poppies. Here the authors show that bacteria can serve as an efficient and flexible platform for the production of opiates by demonstrating the total synthesis of Thebaine and hydrocodone from stepwise...

www.nature.com

 

[Rider420]

The E. coli system developed in this study thus represents a potentially useful platform for the further development aimed at the industrial production of opiates.

Same old issue of confirmation bias.

Scientists, however, recently reported engineering yeast to carry out the second part of the 15-step opioid-producing reaction. What remained was just the hurdle of coaxing yeast to carry out the first part.

That is what scientists, led by Dr. John Dueber of the University of California, Berkeley, have accomplished.


But with improvements that are well within reach, that dose could be obtained from "a glass of yeast culture grown with sugar on a windowsill," said.

Note this was done in 2015 eight years ago! And opioid users are everywhere lawyers judges and yes even scientists.

 

[Rider420]

The E. coli opiate production system developed in this study does not require specific substrates and resulted in 2.1 mg l−1 thebaine production, which is a 300-fold improvement compared with the reported yeast system

"But with improvements that are well within reach, that dose could be obtained from "a glass of yeast culture grown with sugar on a windowsill,". That is a 600 fold improvement.